Bacteria-Based Production of Thiol-Clickable, Genetically Encoded Lipid Nanovesicles
| Autor: | Jorge Royes, Marta Bally, Christophe Tribet, Federica Angius, Quentin Lubart, Bruno Miroux, Galina V. Dubacheva, Oana Ilioaia |
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| Rok vydání: | 2019 |
| Předmět: |
Proteolipids
Dispersity 010402 general chemistry 01 natural sciences Catalysis chemistry.chemical_compound Adenosine Triphosphate Escherichia coli Sulfhydryl Compounds Maleimide chemistry.chemical_classification Liposome 010405 organic chemistry Vesicle Escherichia coli Proteins General Chemistry Combinatorial chemistry Lipids Transmembrane protein 0104 chemical sciences Proton-Translocating ATPases chemistry Click chemistry Thiol Surface modification Nanoparticles |
| Zdroj: | Angewandte Chemie (International ed. in English). 58(22) |
| ISSN: | 1521-3773 |
| Popis: | Despite growing research efforts on the preparation of (bio)functional liposomes, synthetic capsules cannot reach the densities of protein loading and the control over peptide display that is achieved by natural vesicles. Herein, a microbial platform for high-yield production of lipidic nanovesicles with clickable thiol moieties in their outer corona is reported. These nanovesicles show low size dispersity, are decorated with a dense, perfectly oriented, and customizable corona of transmembrane polypeptides. Furthermore, this approach enables encapsulation of soluble proteins into the nanovesicles. Due to the mild preparation and loading conditions (absence of organic solvents, pH gradients, or detergents) and their straightforward surface functionalization, which takes advantage of the diversity of commercially available maleimide derivatives, bacteria-based proteoliposomes are an attractive eco-friendly alternative that can outperform currently used liposomes. |
| Databáze: | OpenAIRE |
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