Lycium barbarum polysaccharides improve hepatic injury through NFkappa-B and NLRP3/6 pathways in a methionine choline deficient diet steatohepatitis mouse model
| Autor: | Jia Xiao, George L. Tipoe, Emily C. Liong, Kwok-Fai So, Fei Wang |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Antioxidant medicine.medical_treatment Inflammation Apoptosis Receptors Cell Surface Pharmacology medicine.disease_cause Biochemistry Antioxidants Choline 03 medical and health sciences Mice 0302 clinical medicine Methionine Structural Biology Fibrosis Non-alcoholic Fatty Liver Disease NLR Family Pyrin Domain-Containing 3 Protein medicine Animals Receptor Molecular Biology business.industry NF-kappa B nutritional and metabolic diseases Inflammasome General Medicine medicine.disease nervous system diseases Diet Disease Models Animal Oxidative Stress 030104 developmental biology Liver 030220 oncology & carcinogenesis Female medicine.symptom Steatohepatitis business Oxidative stress medicine.drug Drugs Chinese Herbal |
| Zdroj: | International journal of biological macromolecules. 120 |
| ISSN: | 1879-0003 |
| Popis: | Lycium barbarum polysaccharides (LBP) are major bioactive constituents of wolfberry which possess several pharmacological effects such as antioxidant and immunomodulatory activities. We aimed to evaluate how LBP attenuated the hepatic injury in a non-alcoholic steatohepatitis (NASH) methionine-choline deficient (MCD) mouse model. NASH was induced in C57BL/6N mice by feeding with MCD diet for 6 weeks. During the experiments, 1 mg/kg LBP was intragastrically fed on a daily basis with or without MCD diet lasting from the 4th to 6th week. Control and vehicle–control (LBP + PBS) were fed with a regular animal chow. LBP significantly ameliorated NASH-induced injuries, including the increase of serum ALT and AST levels, hepatic oxidative stress, fibrosis, inflammation, and apoptosis. The hepatoprotective effects of LBP were accompanied by the attenuation of thioredoxin interacting protein, nod-like receptor protein 3/6 (NLRP3/6) and reduced NF-κB (nuclear factor-kappa B) activity. Vehicle LBP fed mice showed no adverse effect on the liver. In conclusion, the suppression of the NLRP3/6 inflammasome pathway and NF-κB activation may partly contribute to the reduction of the hepatic injury during the progression of NASH by therapeutic LBP treatment. |
| Databáze: | OpenAIRE |
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