D-amino-acid oxidase is not present in the mouse liver
| Autor: | Ryuichi Konno, Setsuko Asakura, Masato Sasaki, Akira Niwa, Kiyoshi Fukui, Jumpei Enami |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
D-Amino-Acid Oxidase
Blotting Western Biophysics D-amino acid oxidase Mice Inbred Strains Biology Kidney Biochemistry Polymerase Chain Reaction Mice Complementary DNA medicine Animals Northern blot Molecular Biology Lung Antiserum Oxidase test Myocardium Brain Blotting Northern Molecular biology Blot Reverse transcription polymerase chain reaction medicine.anatomical_structure Liver |
| Zdroj: | Biochimica et biophysica acta. 1335(1-2) |
| ISSN: | 0006-3002 |
| Popis: | Since there are conflict reports on the presence of D-amino-acid oxidase in the mouse liver, this problem was examined. D-Amino-acid oxidase activity was not detected in the homogenates of the mouse liver, lung, or heart, whereas it was detected in the homogenates of the mouse kidney and brain. Western blotting showed that a protein which reacted with the antiserum against pig D-amino-acid oxidase was present in the homogenates of the mouse kidney and brain but not in those of the liver or heart. Northern hybridization using a D-amino-acid oxidase cDNA probe detected a hybridizing signal in poly(A)+ RNAs extracted from the mouse kidney and brain but not in those from the liver, heart, or lung. Reverse transcription-polymerase chain reaction using three primer pairs always amplified D-amino-acid oxidase cDNA fragments of expected sizes in the mouse kidney and brain but very rarely did so in the liver, heart, or lung. The results indicate that D-amino-acid oxidase is not present in the mouse liver in a measurable amount. |
| Databáze: | OpenAIRE |
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