Sensitive visual detection of intracellular zinc ions based on signal-on polydopamine carbon dots
| Autor: | Jiatong Ni, Lixiang Kong, Minglu Tang, Yan Song, Junge Zhao, Wenxin Wang, Tiedong Sun, Ying Wang, Lei Wang |
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| Rok vydání: | 2022 |
| Předmět: |
Ions
Aldehydes Indoles Cell Survival Polymers Mechanical Engineering Bioengineering General Chemistry Carbon Fluorescence Molecular Imaging Zinc Limit of Detection Mechanics of Materials Quantum Dots Humans General Materials Science Single-Cell Analysis Electrical and Electronic Engineering HeLa Cells |
| Zdroj: | Nanotechnology. 33:185502 |
| ISSN: | 1361-6528 0957-4484 |
| Popis: | The concentration of intracellular zinc ions is a significant clinical parameter for diagnosis. However, it is still a challenge for direct visual detection of zinc ions in cells at single-cell level. To address this issue, herein, water-soluble amino-rich polydopamine carbon quantum dots (PDA-CQDs) were successfully synthesized, with strong blue-green fluorescence as the probes for zinc ions detection in cells. The structure and properties of PDA-CQDs were confirmed by transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transformed infrared (FT-IR), UV–visible spectrophotometry (UV–vis), and fluorescence spectroscopy. Importantly, by successfully linking salicylaldehyde (SA) to PDA-CQDs via nucleophilic reaction, the FL quenching and Zn ions induced FL-recovering system was built up, thus offering a signal-on platform for the detection of zinc ions. This PDA-CQDs-SA nanoprobe can be applied for the detection of Zn2+ with a detection limit of 0.09 μM, with good biocompatibility confirmed using cytotoxicity assay. Of significance, the results of fluorescence bioimaging showed that PDA-CQDs-SA is able to detect Zn2+ in single-cell visually, with the detection limit of Zn ions in cells as low as 0.11 μM per cell, which was confirmed using flow cytometry. Therefore, this work offers a potential probe for Zn2+ detection in cells at single-cell level, towards the precise diagnosis of zinc ions related diseases. |
| Databáze: | OpenAIRE |
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