Bioglass/PLGA associated to photobiomodulation: effects on the healing process in an experimental model of calvarial bone defect
| Autor: | Cintia Pereira de Góes, Kelly Rossetti Fernandes, Paulo Roberto Gabbai-Armelin, Gabriela Sodano Fernandes, José Lucas Dos Santos Prado, Angela Maria Paiva Magri, Stephanie de Souza Fermino, Franscisco José Correa Braga, Ana Claudia Muniz Renno, Renata Neves Granito, Hueliton Wilian Kido |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Ceramics Materials science Light 0206 medical engineering Biomedical Engineering Biophysics chemical and pharmacologic phenomena Bioengineering macromolecular substances 02 engineering and technology Bone tissue Biomaterials chemistry.chemical_compound Fractures Bone Tissue engineering In vivo Osteogenesis Materials Testing medicine Animals Rats Wistar Cementation Wound Healing Bone Transplantation Tissue Engineering Experimental model fungi Skull technology industry and agriculture Biomaterial Granulation tissue Phototherapy 021001 nanoscience & nanotechnology Bone defect 020601 biomedical engineering Combined Modality Therapy Rats PLGA medicine.anatomical_structure chemistry Bone Substitutes 0210 nano-technology Polyglycolic Acid Biomedical engineering |
| Zdroj: | Journal of materials science. Materials in medicine. 30(9) |
| ISSN: | 1573-4838 |
| Popis: | Bioactive glasses (BG) are known for their ability to bond to bone tissue. However, in critical situations, even the osteogenic properties of BG may be not enough to induce bone consolidation. Thus, the enrichment of BG with polymers such as Poly (D, L-lactic-co-glycolic) acid (PLGA) and associated to photobiomodulation (PBM) may be a promising strategy to promote bone tissue healing. The aim of the present study was to investigate the in vivo performance of PLGA supplemented BG, associated to PBM therapy, using an experimental model of cranial bone defect in rats. Rats were distributed in 4 different groups (Bioglass, Bioglass/PBM, Bioglas/PLGA and BG/PLGA/PBM). After the surgical procedure to induce cranial bone defects, the pre-set samples were implanted and PBM treatment (low-level laser therapy) started (808 nm, 100 mW, 30 J/cm2). After 2 and 6 weeks, animals were euthanized, and the samples were retrieved for the histopathological, histomorphometric, picrosirius red staining and immunohistochemistry analysis. At 2 weeks post-surgery, it was observed granulation tissue and areas of newly formed bone in all experimental groups. At 6 weeks post-surgery, BG/PLGA (with or without PBM) more mature tissue around the biomaterial particles. Furthermore, there was a higher deposition of collagen for BG/PLGA in comparison with BG/PLGA/PBM, at second time-point. Histomorphometric analysis demonstrated higher values of BM.V/TV for BG compared to BG/PLGA (2 weeks post-surgery) and N.Ob/T.Ar for BG/PLGA compared to BG and BG/PBM (6 weeks post-surgery). This current study concluded that the use of BG/PLGA composites, associated or not to PBM, is a promising strategy for bone tissue engineering. |
| Databáze: | OpenAIRE |
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