Dynamic changes to claudins in the uterine epithelial cells of the marsupial Sminthopsis crassicaudata (Dasyuridae) during pregnancy
| Autor: | Alice L. Buddle, Bronwyn M. McAllan, Camilla M. Whittington, Christopher R. Murphy, Michael B. Thompson, Laura A. Lindsay |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Gene isoform Uterus Tight Junctions 03 medical and health sciences 0302 clinical medicine Pregnancy Genetics medicine Animals Claudin Sminthopsis crassicaudata Marsupial 030219 obstetrics & reproductive medicine biology Tight junction Epithelial Cells Embryo Cell Biology biology.organism_classification Cell biology Marsupialia 030104 developmental biology medicine.anatomical_structure Paracellular transport Claudins Female Developmental Biology |
| Zdroj: | Molecular Reproduction and Development. 86:639-649 |
| ISSN: | 1098-2795 1040-452X |
| DOI: | 10.1002/mrd.23140 |
| Popis: | The fluid that surrounds the embryo in the uterus contains important nourishing factors and secretions. To maintain the distinct microenvironment in the uterine lumen, the tight junctions between uterine epithelial cells are remodeled to decrease paracellular movement of molecules and solutes. Modifications to tight junctions between uterine epithelial cells is a common feature of pregnancy in eutherian mammals, regardless of placental type. Here we used immunofluorescence microscopy and western blot analysis to describe distributional changes to tight junctional proteins, claudin-1, -3, -4, and -5, in the uterine epithelial cells of a marsupial species, Sminthopsis crassicaudata. Immunofluorescence microscopy revealed claudin-1, -3, and -5 in the tight junctions of the uterine epithelium of S. crassicaudata during pregnancy. These specific claudins are associated with restricting passive movement of fluid between epithelial cells in eutherians. Hence, their function during pregnancy in S. crassicaudata may be to maintain the uterine luminal content surrounding developing embryos. Claudin-4 disappears from all uterine regions of S. crassicaudata at the time of implantation, in contrast with the distribution of this claudin in some eutherian mammals. We conclude that like eutherian mammals, distributional changes to claudins in the uterine epithelial cells of S. crassicaudata are necessary to support pregnancy. However, the combination of individual claudin isoforms in the tight junctions of the uterine epithelium of S. crassicaudata differs from that of eutherian mammals. Our findings suggest that the precise permeability of the paracellular pathway of the uterine epithelium is species-specific. |
| Databáze: | OpenAIRE |
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