Efficient expansion of mesenchymal stromal cells in a disposable fixed bed culture system
| Autor: | Kamilla Swiech, Amanda Mizukami, Sâmia Rigotto Caruso, Karen de Lima Prata, Maristela Delgado Orellana, Dimas Tadeu Covas |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Cellular differentiation Cell Culture Techniques Cell Count Umbilical Cord Cell therapy Laboratory flask Bioreactors Bioreactor Humans CD90 Cell Proliferation CÉLULAS-TRONCO business.industry Chemistry Cell growth Mesenchymal stem cell Infant Newborn Microcarrier Cell Differentiation Mesenchymal Stem Cells Biotechnology Cell biology Culture Media Female business Biomarkers |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1520-6033 |
| Popis: | The need for efficient and reliable technologies for clinical-scale expansion of mesenchymal stromal cells (MSC) has led to the use of disposable bioreactors and culture systems. Here, we evaluate the expansion of cord blood-derived MSC in a disposable fixed bed culture system. Starting from an initial cell density of 6.0 × 10(7) cells, after 7 days of culture, it was possible to produce of 4.2(±0.8) × 10(8) cells, which represents a fold increase of 7.0 (±1.4). After enzymatic retrieval from Fibra-Cell disks, the cells were able to maintain their potential for differentiation into adipocytes and osteocytes and were positive for many markers common to MSC (CD73, CD90, and CD105). The results obtained in this study demonstrate that MSC can be efficiently expanded in the culture system. This novel approach presents several advantages over the current expansion systems, based on culture flasks or microcarrier-based spinner flasks and represents a key element for MSC cellular therapy according to GMP compliant clinical-scale production system. |
| Databáze: | OpenAIRE |
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