Development of SYBR green RT-qPCR assay for titrating bivalent live infectious bronchitis vaccines

Autor: Huiming, Yang, Kaihang, Tu, Ye, Zhao, Lu, Sun, Jing, Zhao, Guozhong, Zhang
Rok vydání: 2023
Předmět:
Zdroj: Journal of Virological Methods. 313:114675
ISSN: 0166-0934
Popis: Infectious bronchitis (IB) is a highly contagious viral disease of chickens caused by IB virus (IBV) that can cause substantial economic losses in the poultry industry. IBV variant infections have been continuously reported since the initial description in the 1930s. QX-like IBVs are the predominant circulating genotype globally. A homologous QX vaccine has superior protection efficacy compared with that of other available vaccines, and the combination of Massachusetts (Mass)-like and QX-like strains is being used to combat QX-like IBV infections. Inoculation of embryonated chicken eggs is the standard method for the titration of IBV, and the titer is expressed as 50% egg infectious dose (EID
Databáze: OpenAIRE