Diagnosis of spacer-associated infection using quantitative cultures from sonicated antibiotics-loaded spacers: implications for the clinical outcome
| Autor: | Enrique Sandoval, Laura Prieto-Borja, Joaquín García-Cañete, Jaime Esteban, J Cordero-Ampuero, C. Pérez-Jorge, R. Fernández-Roblas, Antonio Blanco, Ignacio Gadea |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Microbiology (medical) medicine.medical_specialty Pathology Prosthesis-Related Infections medicine.drug_class Arthroplasty Replacement Hip Sonication Antibiotics Periprosthetic Biology Microbiology 03 medical and health sciences 0302 clinical medicine Medical microbiology medicine Humans Prospective Studies 030212 general & internal medicine Prosthesis-Related Infection Arthroplasty Replacement Knee Prospective cohort study Aged Aged 80 and over 030222 orthopedics Arthroplasty Replacement Elbow General Medicine Middle Aged Anti-Bacterial Agents Bacterial Typing Techniques Treatment Outcome Infectious Diseases Colony count Positive culture Female |
| Zdroj: | European Journal of Clinical Microbiology & Infectious Diseases. 35:207-213 |
| ISSN: | 1435-4373 0934-9723 |
| Popis: | Recent studies showed that a positive microbiological result from sonication of the PMMA spacer was associated with poor outcome of patients, but no quantitative analysis has yet been performed. For this purpose, a prospective analysis of 50 spacers (46 patients) was performed. All spacers were processed according to a previously described protocol, including centrifugation and quantitative culture. Clinical data and outcome were also analysed. A statistical relationship between the results of the cultures and the outcome of the patient was assessed. Sixteen patients were diagnosed with spacer-associated infection. Thirteen out of 50 spacers gave a positive culture. Nine of 13 presented with growth of an organism not isolated in the first-stage cultures, and in 7 out of 13 the organisms count was high (>10,000 CFU/ml). We have detected a significant statistical relationship between poor outcome and positive cultures, high colony counts, isolation of different organisms, positive periprosthetic cultures and spacer-associated infection. The detection in a sonicated, antibiotic-loaded PMMA spacer of organisms other than those isolated in the first surgical samples or high colony counts of any organisms is diagnostic with regard to spacer-associated infection. |
| Databáze: | OpenAIRE |
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