Hemocyte migration and expression of four Sox genes during wound healing in Pacific abalone, Haliotis discus hannai
| Autor: | Mugen Koyama, Satoshi Kimura, Kenta Adachi, Takuji Oda, Toyoji Kaneko, Shunsuke Moriyama, Suehiro Furukawa, Kazuma Mori, Gin Sodeyama, Yumi Doshimo, Sei-ichi Okumura, Ryota Inadama, Yuichi Mori, Shohei Funayama, Fumiya Furukawa |
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| Rok vydání: | 2021 |
| Předmět: |
Wound Healing
Hemocytes biology Abalone Gastropoda Cell Gene Expression Cell migration General Medicine Aquatic Science biology.organism_classification Cell biology Proliferating cell nuclear antigen Hemocyte migration medicine.anatomical_structure Cell Movement Proliferating Cell Nuclear Antigen medicine Haliotis discus biology.protein Animals Environmental Chemistry Wound healing Gene SOX Transcription Factors |
| Zdroj: | Fish & Shellfish Immunology. 117:24-35 |
| ISSN: | 1050-4648 |
| DOI: | 10.1016/j.fsi.2021.07.011 |
| Popis: | In molluscs, migration of hemocytes and epithelial cells is believed to play central roles in wound healing. Here, we assessed cellular and molecular mechanisms of wound healing in Pacific abalone, a marine gastropod. Light and electron microscopy in the wounds showed early accumulation of putative hemocytes, collagen deposition by fibroblasts, and further coverage of this tissue by migration of adjacent epithelial cells. Cell labelling technique allowed us to track hemocytes, which migrated to wound surface within 24 h. The migrated cells first expressed PCNA and SoxF weakly, and then the epithelial cells expressed abundant PCNA and SoxB1, SoxB2, and SoxC. These findings imply that abalone SoxF is involved in hemocyte migration or their differentiation into fibroblasts, and suggest that the migrated epithelia acquire stem cell-like property and undergo active proliferation. This study is the first to show direct evidence of hemocyte migration to wounds and expression of Sox genes in molluscan wound healing. |
| Databáze: | OpenAIRE |
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