Characterization of auxin transporter PIN6 plasma membrane targeting reveals a function for PIN6 in plant bolting
Autor: | Tímea Virág Nádai, Beáta Barnabás, Franck Anicet Ditengou, Beata Izabela Ditengou, Hugues Nziengui, Ivan A. Paponov, Violante Medeiros, Philip Kochersperger, Szilvia K. Nagy, Dulceneia Gomes, Katja Rapp, Claude Becker, Tamás Mészáros, Linlin Qi, Róbert Dóczi, Klaus Palme, Hanna Lasok, Chuanyou Li, Xugang Li |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Physiology Meristem Mutant Arabidopsis Plant Science Endoplasmic Reticulum 03 medical and health sciences Gene Expression Regulation Plant Loss of Function Mutation Auxin Arabidopsis thaliana Endomembrane system Inflorescence Phosphorylation chemistry.chemical_classification Bolting Indoleacetic Acids biology Arabidopsis Proteins Chemistry Cell Membrane Membrane Transport Proteins food and beverages Plants Genetically Modified biology.organism_classification Subcellular localization Hypocotyl Cell biology Protein Transport Phosphothreonine 030104 developmental biology Hydrophobic and Hydrophilic Interactions Subcellular Fractions |
Zdroj: | New Phytologist. 217:1610-1624 |
ISSN: | 0028-646X |
DOI: | 10.1111/nph.14923 |
Popis: | Summary Auxin gradients are sustained by series of influx and efflux carriers whose subcellular localization is sensitive to both exogenous and endogenous factors. Recently the localization of the Arabidopsis thaliana auxin efflux carrier PIN-FORMED (PIN) 6 was reported to be tissue-specific and regulated through unknown mechanisms. Here, we used genetic, molecular and pharmacological approaches to characterize the molecular mechanism(s) controlling the subcellular localization of PIN6. PIN6 localizes to endomembrane domains in tissues with low PIN6 expression levels such as roots, but localizes at the plasma membrane (PM) in tissues with increased PIN6 expression such as the inflorescence stem and nectary glands. We provide evidence that this dual localization is controlled by PIN6 phosphorylation and demonstrate that PIN6 is phosphorylated by mitogen-activated protein kinases (MAPKs) MPK4 and MPK6. The analysis of transgenic plants expressing PIN6 at PM or in endomembrane domains reveals that PIN6 subcellular localization is critical for Arabidopsis inflorescence stem elongation post-flowering (bolting). In line with a role for PIN6 in plant bolting, inflorescence stems elongate faster in pin6 mutant plants than in wild-type plants. We propose that PIN6 subcellular localization is under the control of developmental signals acting on tissue-specific determinants controlling PIN6-expression levels and PIN6 phosphorylation. |
Databáze: | OpenAIRE |
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