A Chitosan-PLGA based catechin hydrate nanoparticles used in targeting of lungs and cancer treatment
| Autor: | Hassan Mohammed Ali Almatar, Aftab Alam, Ridha Abdullah Alrasheed, Rizwan Ahmad, Niyaz Ahmad, Taysser Mohammed Buheazah, Hassan Ali Al-Nasif, Hussain Salman AlHomoud, Abdullah Sami Al-Ramadan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Apoptosis CS chitosan Pharmacology 01 natural sciences PVA polyvinyl alcohol Chitosan chemistry.chemical_compound DSC differential scanning calorimetry CC calibration curve IS internal standard lcsh:QH301-705.5 EE entrapment efficiency ANOVA analysis of variance Tmax time to Cmax Chemistry LLOQQC lower limit of quantification for quality control TEM transmission electron microscope CTH Catechin hydrate respiratory system CS-CTH-PLGA-NPs LOD lower limit of detection DCM dichloromethane LC loading capacity PLGA Kel elimination rate constant Toxicity LLOQ liquid–liquid extraction: LLE: lower limit of quantification CH-S catechin-hydrate-suspension Lung cancer General Agricultural and Biological Sciences Quercetin SEM scanning electron microscope Cmax PBS phosphate buffered solution macromolecular substances AUC area under curve CS-CTH-PLGA-NPs chitosan-coated catechin hydrate-loaded-PLGA-nanoparticles UHPLC-MS/MS ultra high performance liquid chromatography mass spectroscopy and mass spectroscopy Article Cmax maximum plasma concentration MQC low quality control: LQC: middle quality control 03 medical and health sciences medicine NPs nanoparticles Lungs comparative pulmokinetics LOQ lower limit of quantitation technology industry and agriculture Cancer PDI polydispersity index t½ half-life medicine.disease Catechin hydrate Bioavailability 030104 developmental biology lcsh:Biology (General) UHPLC-MS/MS ESI Electrospray ionization HQC high quality control Cancer cell 010606 plant biology & botany |
| Zdroj: | Saudi Journal of Biological Sciences, Vol 27, Iss 9, Pp 2344-2357 (2020) Saudi Journal of Biological Sciences |
| Popis: | Objective: To prepare a novel Chitosan (CS)-coated-PLGA-NPs of catechin hydrate (CTH) and to improve lungs bioavailability via direct nose to lungs-delivery for the comparative assessment of a pulmokinetics study by the first-time UHPLC-MS/MS developed method in the treatment of lungs cancer via anticancer activities on H1299 lung cancer cells. Material and methods: PLGA-NPs was prepared by solvent evaporation (double emulsion) method followed by coated with chitosan (CS) and evaluated based on release and permeation of drug, a comparative pulmokinetics study with their anticancer activities on H1299 lung cancer cells. Results: The particle size, PDI and ZP of the optimized CAT-PLGA-NPs and CS-CAT-PLGA-NPs were determined 124.64 ± 12.09 nm and 150.81 ± 15.91 nm, 0.163 ± 0.03 and 0.306 ± 0.03, –3.94 ± 0.19 mV and 26.01 ± 1.19 mV respectively. Furthermore, higher entrapment efficiency was observed for CS-CAT PLGA NPs. The release pattern of the CS-CAT-PLGA NPs was found to favor the release of entrapped CAT within the cancer microenvironment. CS-CAT-PLGA-NPs exposed on H1299 cancer cells upto 24.0 h was found to be higher cytotoxic as compared to CAT-solution (CAT-S). CS-CAT-PLGA-NPs showed higher apoptosis of cancer cells after their exposure as compared to CAT-S. CS-CTH-PLGA-NPs showed tremendous mucoadhesive-nature as compared to CTH-S and CS-CTH-PLGA NPs by retention time (RT) of 0.589 min, and m/z of 289.21/109.21 for CTH alongwith RT of 0.613 min and m/z of 301.21/151.21 was found out for IS (internal standard), i.e. Quercetin). Likewise, for 1–1000 ng mL−1 (linear range) of % accuracy (92.01–99.31%) and %CV (inter & intra-day, i.e. 2.14–3.33%) was determined. The improved Cmax with AUC0–24 was observed extremely significant (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|