A strategy for efficient enrichment of steroidal alkaloids from Fritillaria based on fluorinated reversed‐phase stationary phase
| Autor: | Han Zhou, Zhimou Guo, Xinmiao Liang, Yanming Liu, Ting Peng, Shubin Lu, Dongping Yu, Wei Si, Aijin Shen, Yanfang Liu |
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| Rok vydání: | 2021 |
| Předmět: |
Solvent system
Chromatography Reverse-Phase endocrine system Chromatography biology Plant Extracts Chemistry Elution organic chemicals Fritillaria Filtration and Separation biology.organism_classification complex mixtures Analytical Chemistry Fritillaria cirrhosa Alkaloids Tandem Mass Spectrometry Stationary phase Phase (matter) Steroids heterocyclic compounds Steroidal alkaloid Selectivity Hydrophobic and Hydrophilic Interactions |
| Zdroj: | Journal of Separation Science. 44:3441-3449 |
| ISSN: | 1615-9314 1615-9306 |
| DOI: | 10.1002/jssc.202100379 |
| Popis: | Plant-derived alkaloids are bioactive natural ingredients, but their contents are relatively low in plants. Therefore, efficient enrichment of alkaloids is a prerequisite for purification and further pharmacological research. In this study, an efficient and simple strategy for enrichment of steroidal alkaloid in Fritillaria was developed for the first time based on fluorinated reverse-phase stationary phase (FC8HL). Superior selectivity between alkaloids and non-alkaloids was achieved in a non-aqueous system, and a simple solvent system containing low-content additives was applied to elute alkaloids. Key parameters that affected the elution were investigated, including different types of buffer salts and optimized concentrations. The optimized elution system was then applied to selectively enrich alkaloids from five species of Fritillaria. Its practicability was further demonstrated by enrichment of alkaloids from Fritillaria cirrhosa D.Don at a preparative level. This developed method has great potential for other types of hydrophobic alkaloids. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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