Effect of CB2 Stimulation on Gene Expression in Pediatric B-Acute Lymphoblastic Leukemia: New Possible Targets
| Autor: | Francesca Punzo, Maura Argenziano, Chiara Tortora, Alessandra Di Paola, Margherita Mutarelli, Elvira Pota, Martina Di Martino, Daniela Di Pinto, Maria Maddalena Marrapodi, Domenico Roberti, Francesca Rossi |
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| Přispěvatelé: | Punzo, Francesca, Argenziano, Maura, Tortora, Chiara, DI PAOLA, Alessandra, Mutarelli Elvira Pota, Margherita, Di Martino, Martina, Di Pinto, Daniela, Marrapodi, MARIA MADDALENA, Roberti, Domenico, Rossi, Francesca |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Cannabinoids
Blotting Western Organic Chemistry Gene Expression General Medicine Precursor Cell Lymphoblastic Leukemia-Lymphoma Burkitt Lymphoma Catalysis Computer Science Applications Receptor Cannabinoid CB2 Inorganic Chemistry acute lymphoblastic leukemia SUP-B15 cell line endocannabinoid system RNA sequencing CB2 receptors Receptor Cannabinoid CB1 Humans Physical and Theoretical Chemistry Child Molecular Biology SEC Translocation Channels Spectroscopy |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 15; Pages: 8651 |
| Popis: | Acute lymphoblastic leukemia type B (B-ALL) is the most common kind of pediatric leukemia, characterized by the clonal proliferation of type B lymphoid stem cells. Important progress in ALL treatments led to improvements in long-term survival; nevertheless, many adverse long-term consequences still concern the medical community. Molecular and cellular target therapies, together with immunotherapy, are promising strategies to overcome these concerns. Cannabinoids, enzymes involved in their metabolism, and cannabinoid receptors type 1 (CB1) and type 2 (CB2) constitute the endocannabinoid system, involved in inflammation, immune response, and cancer. CB2 receptor stimulation exerts anti-proliferative and anti-invasive effects in many tumors. In this study, we evaluated the effects of CB2 stimulation on B-ALL cell lines, SUP-B15, by RNA sequencing, Western blotting, and ELISA. We observe a lower expression of CB2 in SUP-B15 cells compared to lymphocytes from healthy subjects, hypothesizing its involvement in B-ALL pathogenesis. CB2 stimulation reduces the expression of CD9, SEC61G, TBX21, and TMSB4X genes involved in tumor growth and progression, and also negatively affects downstream intracellular pathways. Our findings suggest an antitumor role of CB2 stimulation in B-ALL, and highlight a functional correlation between CB2 receptors and specific anti-tumoral pathways, even though further investigations are needed. |
| Databáze: | OpenAIRE |
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