Dual Fatty Acid Elongase Complex Interactions in Arabidopsis
| Autor: | Yannick Bellec, Johnathan A. Napier, Céline Morineau, Frédéric Beaudoin, Richard P. Haslam, Charlotte Renne, Lionel Gissot, Frédérique Tellier, Kian Hématy, Jean-Denis Faure |
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| Přispěvatelé: | Faure, Jean Denis, Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Rothamsted Research, Ministere de l'Enseignement Superieur et de la Recherche (France), LabEx Saclay Plant Sciences-SPS [ANR-10-LABX-0040-SPS] |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine cell-proliferation Cellular differentiation [SDV]Life Sciences [q-bio] Arabidopsis lcsh:Medicine Artificial Gene Amplification and Extension plant Fatty acid elongase complex Plant Science sphingolipsynthesis Endoplasmic Reticulum Pathology and Laboratory Medicine 01 natural sciences Biochemistry Polymerase Chain Reaction functional-characterization Medicine and Health Sciences division Dehydration (Medicine) lcsh:Science chemistry.chemical_classification Multidisciplinary biology Plant Anatomy Fatty Acids pasticcino genes plasma-membrane Plants Lipids Fatty acid elongation Research Article Fatty Acid Elongases Arabidopsis Thaliana Saccharomyces cerevisiae Brassica secretory pathway thaliana reductase Research and Analysis Methods 03 medical and health sciences Model Organisms Signs and Symptoms Acetyltransferases Plant and Algal Models Diagnostic Medicine Molecular Biology Techniques Molecular Biology Endodermis lcsh:R Organisms Fungi Fatty acid Biology and Life Sciences biology.organism_classification Yeast 030104 developmental biology chemistry Seedlings Dehydratase Mutation Ectopic expression lcsh:Q Cytokinesis 010606 plant biology & botany Cloning |
| Zdroj: | Plos One 9 (11), 1-20. (2016) PLoS ONE PLoS ONE, Public Library of Science, 2016, 11 (9), pp.e0160631. ⟨10.1371/journal.pone.0160631⟩ PLoS ONE, Vol 11, Iss 9, p e0160631 (2016) PLoS ONE, Public Library of Science, 2016, 11, pp.e0160631. ⟨10.1371/journal.pone.0160631⟩ |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0160631⟩ |
| Popis: | International audience; Very long chain fatty acids (VLCFAs) are involved in plant development and particularly in several cellular processes such as membrane trafficking, cell division and cell differentiation. However, the precise role of VLCFAs in these different cellular processes is still poorly understood in plants. In order to identify new factors associated with the biosynthesis or function of VLCFAs, a yeast multicopy suppressor screen was carried out in a yeast mutant strain defective for fatty acid elongation. Loss of function of the elongase 3 hydroxyacyl-CoA dehydratase PHS1 in yeast and PASTICCINO2 in plants prevents growth and induces cytokinesis defects. PROTEIN TYROSIN PHOSPHATASE-LIKE (PTPLA) previously characterized as an inactive dehydratase was able to restore yeast phs1 growth and VLCFAs elongation but not the plant pas2-1 defects. PTPLA interacted with elongase subunits in the Endoplasmic Reticulum (ER) and its absence induced the accumulation of 3-hydroxyacyl-CoA as expected from a dehydratase involved in fatty acid (FA) elongation. However, loss of PTPLA function increased VLCFA levels, an effect that was dependent on the presence of PAS2 indicating that PTPLA activity repressed FA elongation. The two dehydratases have specific expression profiles in the root with PAS2, mostly restricted to the endodermis, while PTPLA was confined in the vascular tissue and pericycle cells. Comparative ectopic expression of PTPLA and PAS2 in their respective domains confirmed the existence of two independent elongase complexes based on PAS2 or PTPLA dehydratase that are functionally interacting. |
| Databáze: | OpenAIRE |
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