Expression of Recombinant Human Coagulation Factor VII by the LizardLeishmaniaExpression System
| Autor: | Kazem Parivar, Sina Mirzaahmadi, Nahid Hosseinzadeh, Bahram Kazemi, Hasan Mirzahoseini, Nooshin Davoudi, Leila Tahmasbi, Mojgan Bandehpour, Golnaz Asaadi-Tehrani |
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| Přispěvatelé: | Department of Biology, Science and Research Branch, West Tehran Islamic Azad University [Tehran] (WTIAU), Cellular and Molecular Biology Research Center (SBU), Shahid Beheshti University of Medical Sciences [Tehran] (SBUMS), Shahid Beheshti University-Shahid Beheshti University, Institut Pasteur d'Iran, Réseau International des Instituts Pasteur (RIIP), Department of Hematology, Shahid Beheshti University-Shahid Beheshti University-Faculty of Paramedical Sciences, Biotechnology Department, Faculty of Medicine-Shahid Beheshti University of Medical Sciences [Tehran] (SBUMS), SinaMirzaahmadi, Golnaz Asaadi-Tehrani, Mojgan Bandehpour, Nooshin Davoudi, Leila Tahmasbi, Nahid Hosseinzadeh, Hasan Mirzahoseini, Kazem Parivar, Bahram Kazemi, Faculty of Paramedical Sciences-Shahid Beheshti University of Medical Sciences [Tehran] (SBUMS) |
| Rok vydání: | 2011 |
| Předmět: |
Models
Molecular [SDV.BIO]Life Sciences [q-bio]/Biotechnology Health Toxicology and Mutagenesis lcsh:Medicine MESH: Lizards 030204 cardiovascular system & hematology Protein Engineering expression system law.invention MESH: Recombinant Proteins chemistry.chemical_compound 0302 clinical medicine law hemic and lymphatic diseases Gene expression MESH: Animals recombinant [INFO.INFO-BT]Computer Science [cs]/Biotechnology Cloning Molecular Blood coagulation test Leishmania 0303 health sciences human coagulation factor VII Factor VII medicine.diagnostic_test Lizards Hep G2 Cells General Medicine Recombinant Proteins MESH: Protein Engineering Blot Recombinant DNA Molecular Medicine Electrophoresis Polyacrylamide Gel MESH: Factor VII Blood Coagulation Tests MESH: Models Molecular Research Article Plasmids Biotechnology Article Subject lcsh:Biotechnology MESH: Leishmania Blotting Western MESH: Hep G2 Cells Biology 03 medical and health sciences Western blot Lizard Leishmania MESH: Plasmids lcsh:TP248.13-248.65 Complementary DNA parasitic diseases Genetics medicine MESH: Blotting Western Animals Humans MESH: Cloning Molecular cardiovascular diseases Molecular Biology 030304 developmental biology MESH: Humans lcsh:R MESH: Blood Coagulation Tests biology.organism_classification Molecular biology chemistry MESH: Electrophoresis Polyacrylamide Gel |
| Zdroj: | Journal of Biomedicine and Biotechnology Journal of Biomedicine and Biotechnology, Hindawi Publishing Corporation, 2011, 2011, pp.873874. ⟨10.1155/2011/873874⟩ Journal of Biomedicine and Biotechnology, Vol 2011 (2011) |
| ISSN: | 1110-7251 1110-7243 |
| DOI: | 10.1155/2011/873874 |
| Popis: | The variety of recombinant protein expression systems have been developed as a resource of FVII gene expression. In the current study, the authors used a novel protein expression system based on the Iranian LizardLeishmania, a trypanosomatid protozoan as a host for expression of FVII. Plasmid containing cDNA encoding full-length human FVII was introduced into LizardLeishmaniaand positive transfectants were analyzed by SDS-PAGE and Western blot analysis. Furthermore, biological activity of purified protein was detected by PT assay. The recombinant strain harboring a construct was analyzed for expression of FVII at the mRNA and protein level. Purified rFVII was obtained and in order to confirm the purified compound was in fact rFVII. Western blot analysis was carried out. Clotting time in PT assay was reduced about 30 seconds with the purified rFVII. In Conclusion, this study has demonstrated, for the first time, thatLeishmaniacells can be used as an expression system for producing recombinant FVII. |
| Databáze: | OpenAIRE |
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