Requirement for glutamine in the expression of alpha-actin and type IV collagen in mesangial cells
| Autor: | Rui Curi, Tania Cristina Pithon-Curi, Marcos M. Machado, Przemyslaw Hirszel, Donald F. Sellitti, Sonia Q. Doi |
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| Rok vydání: | 2005 |
| Předmět: |
Collagen Type IV
Glutamine Clinical Biochemistry Cell Tenascin Gene Expression Biochemistry Extracellular matrix Type IV collagen Mice Gene expression medicine Animals RNA Messenger Cells Cultured chemistry.chemical_classification biology Cell Biology General Medicine Molecular biology Actins Amino acid Extracellular Matrix Fibronectins Fibronectin medicine.anatomical_structure chemistry Mesangial Cells biology.protein |
| Zdroj: | Cell biochemistry and function. 24(3) |
| ISSN: | 0263-6484 |
| Popis: | The importance of glutamine for the synthesis of proteins of the extracellular matrix was investigated in cultured mesangial cells. Glutamine at 2 mM elicited an increase in smooth muscle cell alpha-actin, alpha(1)-type IV collagen and fibronectin transcripts (19.0-, 16.7-, and 4.3-fold, respectively) accompanied by an increase in alpha-actin stress fibres compared to cells grown in absence of glutamine. The specificity for the glutamine requirement is suggested by the fact that mRNA levels of tenascin were not altered by addition of glutamine. This suggests that glutamine is required for the expression of important proteins of the extracellular matrix in cultured mesangial cells. |
| Databáze: | OpenAIRE |
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