Discovery and structure-activity relationships study of thieno[2,3-b]pyridine analogues as hepatic gluconeogenesis inhibitors
| Autor: | Fei Ma, Yinan Zhang, Min Lei, Jian Liu, Lihong Hu, Xu Shen, Tingting Zhou, Xiachang Wang, Jing Chen |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Thienopyridine Pyridines Cell Mice Obese Pharmacology 01 natural sciences Mice Structure-Activity Relationship 03 medical and health sciences Diabetes mellitus Drug Discovery medicine Animals Hypoglycemic Agents Dose-Response Relationship Drug Molecular Structure 010405 organic chemistry Drug discovery Chemistry Organic Chemistry Gluconeogenesis General Medicine Gluconeogenesis Inhibition Glucose Tolerance Test medicine.disease Small molecule 0104 chemical sciences Glucose 030104 developmental biology medicine.anatomical_structure Liver Phosphoenolpyruvate carboxykinase |
| Zdroj: | European Journal of Medicinal Chemistry. 152:307-317 |
| ISSN: | 0223-5234 |
| DOI: | 10.1016/j.ejmech.2018.04.028 |
| Popis: | Type 2 diabetes mellitus (T2DM) is a chronic, complex and multifactorial metabolic disorder, and targeting gluconeogenesis inhibition is a promising strategy for anti-diabetic drug discovery. This study discovered a new class of thieno[2,3-b]pyridine derivatives as hepatic gluconeogenesis inhibitors. First, a hit compound (DMT: IC50 = 33.8 μM) characterized by a thienopyridine core was identified in a cell-based screening of our privileged small molecule library. Structure activity relationships (SARs) study showed that replaced the CF3 in the thienopyridine core could improve the potency and led to the discovery of 8e (IC50 = 16.8 μM) and 9d (IC50 = 12.3 μM) with potent inhibition of hepatic glucose production and good drug-like properties. Furthermore, the mechanism of 8e for the inhibition of hepatic glucose production was also identified, which could be effective through the reductive expression of the mRNA transcription level of gluconeogenic genes, including glucose-6-phosphatase (G6Pase) and hepatic phosphoenolpyruvate carboxykinase (PEPCK). Additionally, 8e could also reduce the fasting blood glucose and improve the oral glucose tolerance and pyruvate tolerance in db/db mice. The optimization of this class of derivatives had provided us a start point to develop new anti-hepatic gluconeogenesis agents. |
| Databáze: | OpenAIRE |
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