The Biosynthesis Characteristics of TTP and TNF Can Be Regulated through a Posttranscriptional Molecular Loop
| Autor: | Naveed Aslam, Irum Zaheer |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Translational efficiency
RNA Stability p38 mitogen-activated protein kinases Immunology Biology Models Biological Biochemistry Mice Transcription (biology) Gene expression Animals Homeostasis Gene silencing Computer Simulation RNA Messenger Molecular Biology Adaptor Proteins Signal Transducing Feedback Physiological Inflammation Mice Knockout Regulation of gene expression Messenger RNA Tumor Necrosis Factor-alpha Cell Biology Molecular biology Cell biology Gene Expression Regulation Tumor necrosis factor alpha |
| Zdroj: | Journal of Biological Chemistry. 286:3767-3776 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m110.168757 |
| Popis: | The abundant expression of tumor necrosis factor (TNF) is a hallmark of chronic inflammation of the gastrointestinal tract. Prolonged inflammation can lead to inflammatory bowel disease. TNF biosynthesis is regulated both at transcription and posttranscriptional levels. However, the stimulation-induced increase in translation rate is much larger. This might indicate the possibility of a posttranscriptional regulatory mechanism. How, during basal conditions, is the free concentration of TNF tightly regulated at low levels? The stability and translational efficiency of TNF transcript are regulated by an AU-rich element (ARE) in the 3'-UTR of messenger RNA. A transacting protein, TTP, binds to ARE and enhances the mRNA turnover. Here, we examine a proposal that TNF homeostasis is regulated by a TTP-TNF interaction loop at the posttranscriptional level. We propose a computational framework of this regulatory loop by modeling the role of AREs in mediating the messenger RNA stability and translation. This posttranscriptional regulatory loop between TTP and TNF is composed of two feedback loops (i.e. positive and negative). The mutual interaction of these feedback loops regulates the biosynthesis response of TNF during basal and inflammatory conditions. Here, we also propose an explanation for why the p38 inhibitors become insensitive for TTP knock-out mice. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|