Optofluidic control of rodent learning using cloaked caged glutamate
| Autor: | Philippe Faure, Pradeep S. Chauhan, Adriana Pétriz Reyes, Alexandre Mourot, Graham C. R. Ellis-Davies, Romain Durand-de Cuttoli |
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| Přispěvatelé: | Neurosciences Paris Seine (NPS), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Biologie Paris Seine (IBPS), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Icahn School of Medicine at Mount Sinai [New York] (MSSM) |
| Rok vydání: | 2020 |
| Předmět: |
biologically inert
Indoles Photochemistry [SDV]Life Sciences [q-bio] Microfluidics 010402 general chemistry Inhibitory postsynaptic potential Receptors N-Methyl-D-Aspartate 01 natural sciences Mice 03 medical and health sciences Glutamates Receptors GABA Dopamine Dendrimer medicine Animals Learning conditioned place-preference Receptor 030304 developmental biology Neurons Neurotransmitter Agents 0303 health sciences GABA-A antagonism Photolysis Multidisciplinary caged glutamate Chemistry optofluidics [SCCO.NEUR]Cognitive science/Neuroscience Glutamate receptor Neurochemistry Conditioned place preference In vitro 0104 chemical sciences Mice Inbred C57BL Ventral tegmental area medicine.anatomical_structure Biophysics medicine.drug |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2020, 117 (12), pp.6831-6835. ⟨10.1073/pnas.1920869117⟩ |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.1920869117 |
| Popis: | International audience; Glutamate is the major excitatory neurotransmitter in the brain, and photochemical release of glutamate (or uncaging) is a chemical technique widely used by biologists to interrogate its physiology. A basic prerequisite of these optical probes is bio-inertness before photolysis. However, all caged glutamates are known to have strong antagonism toward receptors of γ-aminobutyric acid, the major inhibitory transmitter. We have developed a caged glutamate probe that is inert toward these receptors at concentrations that are effective for photolysis with violet light. Pharmacological tests in vitro revealed that attachment of a fifth-generation (G5) dendrimer (i.e., cloaking) to the widely used 4-methoxy-7-nitro-indolinyl(MNI)-Glu probe prevented such off-target effects while not changing the photochemical properties of MNI-Glu significantly. G5-MNI-Glu was used with optofluidic delivery to stimulate dopamine neurons of the ventral tegmental area of freely moving mice in a conditioned place-preference protocol so as to mediate Pavlovian conditioning. |
| Databáze: | OpenAIRE |
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