New cytolysins in sea anemones from the west coast of the United States
| Autor: | Lois S. Avigad, Alan W. Bernheimer |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Lysis
biology Molecular mass Cytotoxins Ecology Anthopleura xanthogrammica Lysin Sea pansy Toxicology biology.organism_classification Hemolysis Lipids United States Molecular Weight Cnidaria Sea Anemones Isoelectric point Biochemistry Epiactis Chromatography Gel Animals Isoelectric Focusing Corynactis |
| Zdroj: | Toxicon. 19:529-534 |
| ISSN: | 0041-0101 |
| Popis: | Seven kinds of sea anemones and the sea pansy, Renilla kollikeri , were examined for the presence of cytolytic toxins. Tealia lofotensis and Epiactis prolfiera yielded very potent lytic preparations whereas extracts of Anthopleura xanthogrammica , A. elegantissima , A. artemisia , Corynactis california , Pachycerianthus torreyi and R. kollikeri showed weaker cytolytic activity. Lysins were examined with respect to lytic potency on erythrocytes from man, sheep, rabbit, dog and horse. Their capacity to be inhibited by the major lipids of the erythrocyte membrane was examined with the finding that sphingomyelin specifically prevented lysis by preparations obtained from E. prolifera and A. xanthogrammica . Lysins of T. lofotensis and R. kollikeri were isoelectric at pH 9·4 and 4·2, respectively. That of A. xanthogrammica electrofocused as two components, one at pH 9·4 and one at pH 4·2. That of E. prolifera also electrofocused as two components, one at pH 6·5 and one at pH 8·3. Molecular weights ranged from approximately 10,000 to > 100,000. The data suggest the existence of a variety of cytolysins in different sea anemones. |
| Databáze: | OpenAIRE |
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