The location of acidic fibroblast growth factor in the breast is dependent on the activity of proteases present in breast cancer tissue
| Autor: | G. S. Bansal, C Yiangou, Sami Shousha, J. J. Gomm, R. C. Coombes, R. C. Coope, C. L. Johnston, P. J. Browne, N. Groome, J. Walters |
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| Jazyk: | angličtina |
| Rok vydání: | 1997 |
| Předmět: |
Cancer Research
medicine.medical_specialty Proteases medicine.medical_treatment Immunoblotting Breast Neoplasms Biology HUMAN MAMMARY-GLAND Fibroblast growth factor MOUSE Polymerase Chain Reaction Paracrine signalling Mice breast cancer Internal medicine Endopeptidases medicine EXTRACELLULAR-MATRIX Animals Humans Receptor Fibroblast Growth Factor Type 4 Receptor Fibroblast Growth Factor Type 1 Oncology & Carcinogenesis Autocrine signalling fibroblast growth factor 1 GENE-EXPRESSION FACTOR RECEPTOR-1 Science & Technology CARCINOMA CELLS Growth factor SIGNAL TRANSDUCTION Myoepithelial cell Receptor Protein-Tyrosine Kinases protease LOCALIZATION Fibroblast growth factor receptor 4 ONCOLOGY FACTOR MESSENGER-RNA Molecular biology Immunohistochemistry Receptors Fibroblast Growth Factor Endocrinology Female Life Sciences & Biomedicine 1112 Oncology And Carcinogenesis Immunostaining FACTOR FAMILY Research Article |
| Zdroj: | British Journal of Cancer |
| Popis: | Acidic fibroblast growth factor (FGF1) and two of its receptors, FGFR1 and FGFR4, were localized in cryostat sections of normal, benign and malignant human breast tissue by immunohistochemistry. Without pretreatment, FGF1 staining was mainly seen in normal epithelial cells. However, polymerase chain reaction (PCR) analysis and immunoblotting of isolated normal epithelial and myoepithelial cells showed FGF1 mRNA and protein to be present in both cell types. Following incubation of frozen sections at 37 degrees C in phosphate-buffered saline, FGF1 staining was also revealed in myoepithelial cells and basement membrane adjacent to carcinoma cells. Treatment with protease inhibitors demonstrated that this effect was due to the activity of an endogenous protease. In contrast, FGF1 staining was found to be associated with the stroma adjacent to malignant cells only in the presence of protease inhibitors. FGFR1 and FGFR4 immunostaining was localized to both normal and malignant epithelial cells and to a lesser extent to myoepithelial cells. There was no difference in the staining intensity for the FGF receptors between normal and cancer samples. The change in location of FGF1 between normal and malignant tissues and the sensitivity of stored FGF1 to the action of endogenous proteases raises the possibility of both autocrine and paracrine roles for FGF1 in the normal and malignant human breast. Images Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 |
| Databáze: | OpenAIRE |
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