In vitro studies of interaction of modified silica nanoparticles with different types of immunocompetent cells
| Autor: | Elena V. Parfenyuk, L. V. Posiseeva, Nataliya Yu. Sotnikova, Yuliya S. Antsiferova, Mariya A. Eliseeva, Galina A. Kulikova, Irina V. Ryabinina |
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| Rok vydání: | 2010 |
| Předmět: |
Materials science
Surface Properties Lymphocyte Biomedical Engineering Nanoparticle Flow cytometry Biomaterials chemistry.chemical_compound Adsorption Materials Testing medicine Animals Humans Macrophage Lymphocytes Particle Size Cells Cultured Fluorescent Dyes Sheep Molecular Structure biology medicine.diagnostic_test Metals and Alloys Flow Cytometry Silicon Dioxide Molecular biology In vitro medicine.anatomical_structure chemistry Immunoglobulin G Isothiocyanate Macrophages Peritoneal Ceramics and Composites biology.protein Biophysics Nanoparticles Female Antibody Fluorescein-5-isothiocyanate |
| Zdroj: | Journal of Biomedical Materials Research Part A. :434-439 |
| ISSN: | 1549-3296 |
| DOI: | 10.1002/jbm.a.32855 |
| Popis: | Interactions between different types of immune cells and organically-modified silica nanoparticles were studied. The silica particles functionalized with amine groups were prepared by sol–gel technique. Sheep immunoglobulin labeled with fluoresceine isothiocyanate was immobilized by adsorption onto the nanoparticles. The presence of the functional groups was confirmed by infrared absorption measurements. The level of immunocompetent cells interacting with the silica nanoparticles was estimated as the amount of fluorescence-bright cells by flow cytometry method. A low level of interaction of the peripheral blood lymphocytes with the silica nanoparticles was found. On the contrary, the macrophages are actively involved in interaction with the silica nanoparticles. The influence of different size of the silica nanoparticles and incubation time on viability and functional activity of peripheral blood lymphocytes and peritoneal macrophages were investigated. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010. |
| Databáze: | OpenAIRE |
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