A transcription factor hierarchy defines an environmental stress response network
| Autor: | Huaming Chen, Aaron Wise, Rosa Castanon, Joseph R. Ecker, Ziv Bar-Joseph, Shao-shan Carol Huang, Liang Song, Jerushah Thomas, Joseph R. Nery, Marina Watanabe |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Chromatin Immunoprecipitation Salinity DNA Plant Arabidopsis Gene regulatory network Computational biology Article 03 medical and health sciences chemistry.chemical_compound Plant Growth Regulators Gene Expression Regulation Plant Osmotic Pressure Stress Physiological Abscisic acid Transcription factor Regulation of gene expression Binding Sites Multidisciplinary biology Arabidopsis Proteins Ecology Systems Biology fungi food and beverages Water Sequence Analysis DNA biology.organism_classification Droughts Chromatin 030104 developmental biology chemistry Plant hormone Chromatin immunoprecipitation Abscisic Acid Protein Binding Transcription Factors |
| Zdroj: | Science. 354 |
| ISSN: | 1095-9203 0036-8075 |
| Popis: | Environmental stresses are universally encountered by microbes, plants and animals. Yet systematic studies of stress-responsive transcription factor (TF) networks in multi-cellular organisms have been limited. The phytohormone abscisic acid (ABA) influences the expression of thousands of genes, allowing us to characterize complex stress-responsive regulatory networks. Using chromatin immunoprecipitation sequencing, we identified genome-wide targets of 21 ABA-related TFs to construct a comprehensive regulatory network in Arabidopsis thaliana. Determinants of dynamic TF binding and a hierarchy among TFs were defined, illuminating the relationship between differential gene expression patterns and ABA pathway feedback regulation. By extrapolating regulatory characteristics of observed canonical ABA pathway components, we identified a new family of transcriptional regulators modulating ABA and salt responsiveness and demonstrated their utility to modulate plant resilience to osmotic stress. |
| Databáze: | OpenAIRE |
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