Decrease in Proportion of CD19+CD24hiCD27+ B Cells and Impairment of Their Suppressive Function in Graves’ Disease
Autor: | Zaoping Chen, Luman Wang, Jiong Xu, Yiwei Chu, Jun Liu, Li Sheng, Bingbing Zha, Yiming Li, Xiaoming Liu |
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Rok vydání: | 2012 |
Předmět: |
CD4-Positive T-Lymphocytes
Male Anatomy and Physiology B Cells medicine.medical_treatment lcsh:Medicine Autoimmunity Lymphocyte Activation Interleukin 21 Endocrinology Immune Physiology lcsh:Science Thyroid B-Lymphocytes Multidisciplinary biology T Cells hemic and immune systems Middle Aged Graves Disease Interleukin-10 Interleukin 10 Phenotype medicine.anatomical_structure Cytokine Cytokines Medicine Female Research Article Adult medicine.medical_specialty Immune Cells T cell Antigens CD19 Immunology chemical and pharmacologic phenomena CD19 Immunophenotyping Autoimmune Diseases Immunomodulation Young Adult Immune system Antigen Internal medicine medicine Humans Biology B cell Autoantibodies lcsh:R CD24 Antigen Tumor Necrosis Factor Receptor Superfamily Member 7 Graves' Disease biology.protein lcsh:Q Clinical Immunology |
Zdroj: | PLoS ONE PLoS ONE, Vol 7, Iss 11, p e49835 (2012) |
ISSN: | 1932-6203 |
Popis: | IL-10-producing B cells (B10 cells) have been shown to play a suppressive role in the peripheral blood of humans, with their numbers and function altered in several autoimmune diseases. However, the role of B10 cells in Graves' disease (GD) remains unknown. In this study, we demonstrated that B10 cells in human peripheral blood belonged to a CD24(hi)CD27(+) B cell subpopulation. The proportion of B10 cells along with the CD19(+)CD24(hi)CD27(+) B cell subset was significantly lower in new-onset patients compared with healthy individuals. In recovered patients, these proportions were restored to levels similar to those seen in healthy individuals. Additionally, we found that CD19(+)CD24(hi)CD27(+) B cells from healthy individuals inhibited proliferation and TNF-α production of CD4(+) T cells via an IL-10-independent pathway. They also inhibited IFN-γ production by CD4(+) T cells, through an IL-10-dependent pathway. In contrast, their suppressive function on CD4(+) T cell proliferation and cytokine production was impaired in new-onset and recovered patients compared with healthy individuals. Our study provides evidence that CD19(+)CD24(hi)CD27(+) B cells may possess the capacity to downregulate immune responses, partially by production of IL-10 in human peripheral blood. Impairment of their immunosuppressive function may contribute to GD pathogenesis and relapse. |
Databáze: | OpenAIRE |
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