Decrease in Proportion of CD19+CD24hiCD27+ B Cells and Impairment of Their Suppressive Function in Graves’ Disease

Autor: Zaoping Chen, Luman Wang, Jiong Xu, Yiwei Chu, Jun Liu, Li Sheng, Bingbing Zha, Yiming Li, Xiaoming Liu
Rok vydání: 2012
Předmět:
CD4-Positive T-Lymphocytes
Male
Anatomy and Physiology
B Cells
medicine.medical_treatment
lcsh:Medicine
Autoimmunity
Lymphocyte Activation
Interleukin 21
Endocrinology
Immune Physiology
lcsh:Science
Thyroid
B-Lymphocytes
Multidisciplinary
biology
T Cells
hemic and immune systems
Middle Aged
Graves Disease
Interleukin-10
Interleukin 10
Phenotype
medicine.anatomical_structure
Cytokine
Cytokines
Medicine
Female
Research Article
Adult
medicine.medical_specialty
Immune Cells
T cell
Antigens
CD19
Immunology
chemical and pharmacologic phenomena
CD19
Immunophenotyping
Autoimmune Diseases
Immunomodulation
Young Adult
Immune system
Antigen
Internal medicine
medicine
Humans
Biology
B cell
Autoantibodies
lcsh:R
CD24 Antigen
Tumor Necrosis Factor Receptor Superfamily
Member 7
Graves' Disease
biology.protein
lcsh:Q
Clinical Immunology
Zdroj: PLoS ONE
PLoS ONE, Vol 7, Iss 11, p e49835 (2012)
ISSN: 1932-6203
Popis: IL-10-producing B cells (B10 cells) have been shown to play a suppressive role in the peripheral blood of humans, with their numbers and function altered in several autoimmune diseases. However, the role of B10 cells in Graves' disease (GD) remains unknown. In this study, we demonstrated that B10 cells in human peripheral blood belonged to a CD24(hi)CD27(+) B cell subpopulation. The proportion of B10 cells along with the CD19(+)CD24(hi)CD27(+) B cell subset was significantly lower in new-onset patients compared with healthy individuals. In recovered patients, these proportions were restored to levels similar to those seen in healthy individuals. Additionally, we found that CD19(+)CD24(hi)CD27(+) B cells from healthy individuals inhibited proliferation and TNF-α production of CD4(+) T cells via an IL-10-independent pathway. They also inhibited IFN-γ production by CD4(+) T cells, through an IL-10-dependent pathway. In contrast, their suppressive function on CD4(+) T cell proliferation and cytokine production was impaired in new-onset and recovered patients compared with healthy individuals. Our study provides evidence that CD19(+)CD24(hi)CD27(+) B cells may possess the capacity to downregulate immune responses, partially by production of IL-10 in human peripheral blood. Impairment of their immunosuppressive function may contribute to GD pathogenesis and relapse.
Databáze: OpenAIRE
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