Characterization of a Primordial Major Capsid-Scaffolding Protein Complex in Icosahedral Virus Shell Assembly

Autor:	Christal R. Davis, Donald Backos, Marc C. Morais, Mair E.A. Churchill, Carlos E. Catalano
Rok vydání:	2022
Předmět:	Protein Folding Capsid Structural Biology Virus Assembly Capsid Proteins Molecular Biology Bacteriophage lambda
Zdroj:	Journal of molecular biology. 434(19)
ISSN:	1089-8638
Popis:	Capsid assembly pathways are strongly conserved in the complex dsDNA viruses, where major capsid proteins (MCP) self-assemble into icosahedral procapsid shells, chaperoned by a scaffolding protein. Without a scaffold, the capsid proteins aggregate and form aberrant structures. This, coupled with the rapid co-polymerization of MCP and scaffolding proteins, has thwarted characterization of the earliest steps in shell assembly. Here we interrogate the structure and biophysical properties of a soluble, assembly-deficient phage lambda major capsid protein, MCP(W308A). The mutant protein is folded, soluble to high concentrations and binds to the scaffolding protein in an apparent SP
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55ab2d4bdc2cd5a5580ecd9867aa0642 https://pubmed.ncbi.nlm.nih.gov/35820453 Zobrazit plný text záznamu Full Text from ScienceDirect