Heparin-derived oligosaccharide inhibits vascular intimal hyperplasia in balloon-injured carotid artery
| Autor: | Jie-Ru Liu, Xin-Chao Yu, Rui Xiong, Shu-Ying He, Hui-Fang Wang, Fei-Fei Liu, Xuan Qian, Dan-Feng Yu, Jie Wu |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Vascular Endothelial Growth Factor A medicine.medical_specialty Intimal hyperplasia Basic fibroblast growth factor Blood lipids Oligosaccharides Vascular Cell Adhesion Molecule-1 030204 cardiovascular system & hematology Biology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Restenosis medicine.artery Internal medicine Drug Discovery medicine Animals Common carotid artery Scavenger receptor Chemokine CCL2 Hyperplasia Heparin General Medicine medicine.disease Vascular endothelial growth factor Reverse transcription polymerase chain reaction 030104 developmental biology Endocrinology Complementary and alternative medicine chemistry cardiovascular system Rabbits Carotid Artery Injuries Tunica Intima ATP Binding Cassette Transporter 1 |
| Zdroj: | Chinese journal of natural medicines. 15(6) |
| ISSN: | 1875-5364 |
| Popis: | The aims of the present study were to determine the effects of heparin-derived oligosaccharides (HDOs) on vascular intimal hyperplasia (IH) in balloon-injured carotid artery and to elucidate the underlying mechanisms of action. An animal model was established by rubbing the endothelia within the common carotid artery (CCA) in male rabbits. The rabbits were fed a high-cholesterol diet. Arterial IH was determined by histopathological changes to the CCA. Serum lipids were detected using an automated biochemical analysis. Expressions of mRNAs for vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), vascular cell adhesion molecule-1 (VCAM-1), monocyte chemoattractant protein-1 (MCP-1), scavenger receptor class B type I (SR-BI), and ATP-binding cassette transporter A1 (ABCA-1) were analyzed using reverse transcription polymerase chain reaction assays. Expressions of VEGF, VCAM-1, MCP-1, SR-BI and ABCA-1 proteins were analyzed by Western blotting. Enzyme-linked immunosorbent assays were used to quantify expression levels of VEGF and bFGF. Our results showed that administration of HDO significantly inhibited CCA histopathology and restenosis induced by balloon injury. The treatment with HDOs significantly decreased the mRNA and protein expression levels of VEGF, bFGF, VCAM-1, MCP-1, and SR-BI in the arterial wall; however, ABCA-1 expression level was elevated. HDO treatment led to a reduction in serum lipids (total cholesterol, triglycerides, high-density and low-density lipoproteins). Our results from the rabbit model indicated that HDOs could ameliorate IH and underlying mechanism might involve VEGF, bFGF, VCAM-1, MCP-1, SR-BI, and ABCA-1. |
| Databáze: | OpenAIRE |
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