Assessment of Topical Therapies for Improving the Optical Clarity Following Stromal Wounding in a Novel Ex Vivo Canine Cornea Model
| Autor: | Gregory S. Schultz, Laura R. Proietto, Daniel J. Gibson, William M. Berkowski, Soojung Seo, Caryn E. Plummer, R. David Whitley |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Haze Stromal cell genetic structures Pyridones Corneal Stroma Visual Acuity Administration Ophthalmic Placebo Real-Time Polymerase Chain Reaction Cornea 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Corneal Opacity Dogs Organ Culture Techniques Re-Epithelialization Transforming Growth Factor beta Ophthalmology medicine Animals Fluorescein Vorinostat Wound Healing Corneal Haze business.industry Anti-Inflammatory Agents Non-Steroidal Connective Tissue Growth Factor Epithelium Corneal General Medicine Pirfenidone Immunohistochemistry eye diseases Actins Histone Deacetylase Inhibitors 030104 developmental biology medicine.anatomical_structure chemistry Models Animal 030221 ophthalmology & optometry Lasers Excimer sense organs business Ex vivo medicine.drug Corneal Injuries |
| Zdroj: | Investigative ophthalmologyvisual science. 59(13) |
| ISSN: | 1552-5783 |
| Popis: | Purpose To evaluate the effect of topical suberanilohydroxamic acid (SAHA) and 5-methyl-1-phenyl-2[1H]-pyridone (pirfenidone) on the degree of corneal haze in the stromal wounded ex vivo canine cornea. Methods Twenty-four corneoscleral rims from normal dogs were uniformly wounded with an excimer laser and placed into culture medium with an air-liquid interface. The control group (n = 8) contained placebo-treated corneas. Treatment group 1 (n = 8) received SAHA topically every 6 hours. Treatment group 2 (n = 8) received pirfenidone topically every 6 hours. Each cornea was fluorescein stained and macrophotographed every 6 hours to assess epithelialization rate. All corneas were also macrophotographed weekly to assess optical clarity (haze). Images were analyzed for differences in pixel intensity between wounded (haze) and unwounded (nonhaze) regions, and haze surface area for each cornea was calculated. Results The mean epithelialization time was 47.25 hours in the control group, 45.00 hours in the SAHA group, and 43.50 hours in the pirfenidone group, revealing no significant difference (P = 0.368). The median difference in pixel intensity between haze and nonhaze areas was 21.5 in the control group, 8.0 in the SAHA group, and 8.0 in the pirfenidone group, which is significant (P < 0.01). The median haze surface area was 12.96 mm2 in the control group, 5.70 mm2 in the SAHA group, and 5.92 mm2 in the pirfenidone group, which is significant (P < 0.01). Conclusions Stromal-wounded ex vivo canine corneas exhibited greater optical clarity when treated with SAHA and pirfenidone than when placebo treated at 21 days. There was no significant difference in epithelialization rate between groups. Corneal contour was correlated with geographic haze distribution. |
| Databáze: | OpenAIRE |
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