Prenatal exposure to ethanol: a specific effect on the H19 gene in sperm

Autor: Christelle Stouder, Emmanuel Somm, Ariane Paoloni-Giacobino
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Male
Spermatogenesis/drug effects/genetics
RNA
Untranslated
Alcohol Drinking
Offspring
RNA
Untranslated/genetics
CpG Islands/drug effects
Gestational Age
Biology
Toxicology
Brain/drug effects/embryology/metabolism
Andrology
Alcohol Drinking/adverse effects
Genomic Imprinting
Mice
Pregnancy
Animals
Ethanol/toxicity
ddc:576.5
Epigenetics
Spermatogenesis
Genomic Imprinting/drug effects
Regulation of gene expression
Genetics
ddc:618
Ethanol
Gene Expression Regulation
Developmental/drug effects
Sperm Count
Brain
Gene Expression Regulation
Developmental
Methylation
DNA Methylation
DNA Methylation/drug effects
Spermatozoa/drug effects/metabolism
Spermatozoa
Sperm
female genital diseases and pregnancy complications
Maternal Exposure
Prenatal Exposure Delayed Effects
DNA methylation
embryonic structures
CpG Islands
RNA
Long Noncoding
Female
Genomic imprinting
Zdroj: Reproductive Toxicology, Vol. 31, No 4 (2011) pp. 507-12
ISSN: 0890-6238
Popis: Alcohol exposure during pregnancy induces a range of disorders in the offspring. Methylation changes in imprinted genes may play a role in the teratogenic effects of alcohol. We evaluated the possible effects of alcohol administration in pregnant mice on the methylation pattern of 5 imprinted genes (H19, Gtl2, Peg1, Snrpn and Peg3) in somatic and sperm cell DNAs of the male offspring. The effects observed were a 3% (p < 0.005) decrease in the number of methylated CpGs of H19 in the F1 offspring sperm, a 4% (p < 0.005) decrease in the number of methylated CpGs of H19 in the F2 offspring brain and a 26% (p < 0.05) decrease in the mean sperm concentration. CpGs 1 and 2 of the H19 CTCF-binding site 2 exhibited significant methylation percentage losses. H19 CTCF-binding sites are important for the regulation of Igf2 gene expression. The hypomethylation of H19 may contribute to the decreased spermatogenesis in the offspring.
Databáze: OpenAIRE
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