CYCLIC NUCLEOTIDES and PROTEIN KINASE ACTIVITY IN THE RAT BRAIN POSTMORTEM
| Autor: | Michael J. Schmidt, John F. Thornberry, Lewis L. Truex |
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| Rok vydání: | 1978 |
| Předmět: |
Male
medicine.medical_specialty Cerebellum Time Factors Stimulation Biology Biochemistry Cellular and Molecular Neuroscience Cyclic nucleotide chemistry.chemical_compound Internal medicine Cyclic AMP medicine Animals Tissue Distribution Nucleotide Protein kinase A Cyclic GMP chemistry.chemical_classification Brain Human brain Enzyme assay Rats medicine.anatomical_structure Endocrinology chemistry Cerebral cortex Postmortem Changes biology.protein Protein Kinases |
| Zdroj: | Journal of Neurochemistry. 31:427-431 |
| ISSN: | 1471-4159 0022-3042 |
| DOI: | 10.1111/j.1471-4159.1978.tb02658.x |
| Popis: | — The components of the cyclic nucleotide system were studied in rat brain at various times after death to determine the stability of the system postmortem. The concentration of cyclic AMP in 4 brain regions was highest 10 min after death and declined to stable levels within 6 h after death. At this time concentrations approximated those normally seen in vitro. The concentration of cyclic GMP in brain regions fell markedly postmortem and was undetectable 6 or 16 h after death. Nor-epinephrine-stimulated cyclic AMP accumulation measured in vitro in slices of the cerebral cortex was the same 10 min and 16 h postmortem. In the cerebellum, however, accumulated levels of cyclic AMP were lower 16 h postmortem, although the degree of stimulation elicited by norepinephrine was the same 10 min and 16 h after death. Cyclic AMP-dependent and independent protein kinase activities were detected in the rat brain at all times after death. There was no change in the cyclic AMP-dependent enzyme activity postmortem, but activity in the absence of cyclic AMP was significantly higher immediately after death compared to 6 or 16 h postmortem. These studies indicate that it should be possible to study the cyclic AMP system in human brain tissue obtained at autopsy. |
| Databáze: | OpenAIRE |
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