Micellar antibiotics of bacillus
| Autor: | Wilfried J. J. Meijer, William T. Ferreira, Karolina Bakun, Mateusz Hess, Sisareuth Tan, Loredana Baccigalupi, Simon M. Cutting, Mikhail Soloviev, James R. G. Adams, Ezio Ricca, Enrico Ferrari, Alain Brisson, María Teresa Rejas, Huynh A. Hong, Hannah Wood |
|---|---|
| Přispěvatelé: | Medical Research Council (UK), Ministerio de Economía y Competitividad (España), Ferreira, W. T., Hong, H. A., Hess, M., Adams, J. R. G., Wood, H., Bakun, K., Tan, S., Baccigalupi, L., Ferrari, E., Brisson, A., Ricca, E., Rejas, M. T., Meijer, W. J. J., Soloviev, M., Cutting, S. M. |
| Rok vydání: | 2021 |
| Předmět: |
medicine.drug_class
Antibiotics Pharmaceutical Science Bacillus Bacillus subtilis Bacillus velezensi Micelle Article Microbiology chemistry.chemical_compound Pharmacy and materia medica Chlorotetaine Bacillus velezensis medicine biology Chemistry Lipopeptide Biosurfactant Antimicrobial biology.organism_classification C510 Applied Microbiology RS1-441 Drug delivery Bacteria |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Pharmaceutics Volume 13 Issue 8 Pharmaceutics, Vol 13, Iss 1296, p 1296 (2021) |
| Popis: | Members of the Bacillus genus, particularly the “Bacillus subtilis group”, are known to produce amphipathic lipopeptides with biosurfactant activity. This includes the surfactins, fengycins and iturins that have been associated with antibacterial, antifungal, and anti-viral properties. We have screened a large collection of Bacillus, isolated from human, animal, estuarine water and soil samples and found that the most potent lipopeptide producers are members of the species Bacillus velezensis. B. velezensis lipopeptides exhibited anti-bacterial activity which was localised on the surface of both vegetative cells and spores. Interestingly, lipopeptide micelles (6–10 nm diameter) were detectable in strains exhibiting the highest levels of activity. Micelles were stable (heat and gastric stable) and shown to entrap other antimicrobials produced by the host bacterium (exampled here was the dipeptide antibiotic chlorotetaine). Commercially acquired lipopeptides did not exhibit similar levels of inhibitory activity and we suspect that micelle formation may relate to the particular isomeric forms produced by individual bacteria. Using naturally produced micelle formulations we demonstrated that they could entrap antimicrobial compounds (e.g., clindamycin, vancomycin and resveratrol). Micellar incorporation of antibiotics increased activity. Bacillus is a prolific producer of antimicrobials, and this phenomenon could be exploited naturally to augment antimicrobial activity. From an applied perspective, the ability to readily produce Bacillus micelles and formulate with drugs enables a possible strategy for enhanced drug delivery. Medical Research Council (MR/R026262/1) to SMCand the Ministry of Economy and Competitiveness of the Spanish Government PID2019_108778GB_C21 (AEI/FEDER, EU) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|