In vitro metabolism of chlorpheniramine in the rabbit
| Autor: | Kammerer Rc, Lampe Ma |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Male
Chlorpheniramine Cytochrome Acylation Deamination Biology In Vitro Techniques Biochemistry Gas Chromatography-Mass Spectrometry chemistry.chemical_compound Animals heterocyclic compounds Pharmacology organic chemicals Proadifen Cytochrome P450 Metabolism In vitro Metabolic pathway chemistry biology.protein Microsome Microsomes Liver Rabbits |
| Zdroj: | Biochemical pharmacology. 36(20) |
| ISSN: | 0006-2952 |
| Popis: | The in vitro metabolism of 3-(p-chlorophenyl)-3-(2-pyridyl)-N, N-dimethylpropylamine (chlorpheniramine, I) by rabbit liver microsomes was examined. The metabolites, tentatively identified by gas-liquid chromatography-mass spectrometry, included the mono- and didemethyl metabolites, the aldehyde that results from deamination, and further metabolites of this aldehyde including its intramolecular cyclization product, an indolizine, and its reduction product, the alcohol. Inhibition of metabolism of I by N2, CO, SKF-525A, 2,4-dichloro-6-phenylphenoxyethylamine (DPEA), or deletion of NADPH implies some involvement of cytochrome P-450 in the metabolic reactions. Quantitation of metabolism in these studies accounted for only 69% of the dose, so that binding and/or other undetected metabolic pathways were operative. |
| Databáze: | OpenAIRE |
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