A murine model of appendicitis and the impact of inflammation on appendiceal lymphocyte constituents
| Autor: | Michael Grimm, Andrew R. Lloyd, W S Watson Ng, T Hampartzoumian |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Male
Lymphocyte Immunology Inflammation Appendix Systemic inflammation Immunophenotyping Mice T-Lymphocyte Subsets medicine Animals Immunology and Allergy IL-2 receptor Intestinal Mucosa Cecum Immunity Mucosal Mice Inbred BALB C business.industry Age Factors FOXP3 T lymphocyte Appendicitis medicine.disease Lymphocyte Subsets Specific Pathogen-Free Organisms Disease Models Animal C-Reactive Protein medicine.anatomical_structure Animal Studies medicine.symptom business Serositis CD8 |
| Zdroj: | Clinical and Experimental Immunology. 150:169-178 |
| ISSN: | 1365-2249 0009-9104 |
| DOI: | 10.1111/j.1365-2249.2007.03463.x |
| Popis: | Summary Data indicate that appendicectomy for intra-abdominal inflammation protects against inflammatory bowel disease (IBD). This suggests an important role for the appendix in mucosal immunity. There is no established model of appendicitis. We therefore developed a murine model of appendicitis and examined the effect of inflammation on appendiceal lymphocyte constituents. The caecal patch of specific pathogen-free (SPF)-Balb/c mice was transformed into an obstructed ‘appendiceal pouch’ by standardized suction and band ligation. Mice were killed and ‘pouches’ removed for histology and phenotypic analysis of leucocytes by flow cytometry. Serum C-reactive protein (CRP) was determined by enzyme-linked immunosorbent assay. All ‘pouches’ developed features resembling human appendicitis – mucosal ulceration, transmural inflammation with neutrophils, lymphocytes and occasional eosinophils, and serositis. These changes were most evident between days 7 and 10. There was significant elevation of serum CRP (8·0 ± 0·3 ng/ml to 40·0 ± 3·1 ng/ml; P |
| Databáze: | OpenAIRE |
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