Trait to gene analysis reveals that allelic variation in three genes determines seed vigour

Autor: Peter Glen Walley, William E. Finch-Savage, James R. Lynn, Guy C. Barker, Karl Morris
Rok vydání: 2016
Předmět:
0106 biological sciences
0301 basic medicine
Chromosomes
Artificial
Bacterial
Candidate gene
QTL analysis
Transcription
Genetic
Physiology
Arabidopsis
Gene Dosage
Plant Science
01 natural sciences
chemistry.chemical_compound
Protein Isoforms
Abscisic acid
2. Zero hunger
seed vigour
Full Paper
biology
food and beverages
Full Papers
allelic variation
Physical Chromosome Mapping
Adaptation
Physiological
Phenotype
Germination
Seeds
abscisic acid (ABA)
Brassica oleracea
Genetic Markers
Quantitative Trait Loci
Locus (genetics)
Brassica
Quantitative trait locus
Genes
Plant
Chromosomes
Plant
03 medical and health sciences
Quantitative Trait
Heritable
Transformation
Genetic
Stress
Physiological
Botany
Hybrid Vigor
SB
Gene
Alleles
Arabidopsis Proteins
Research
QK
biology.organism_classification
Alternative Splicing
Mutagenesis
Insertional
030104 developmental biology
chemistry
Seedling
Abscisic Acid
010606 plant biology & botany
Zdroj: The New Phytologist
ISSN: 1469-8137
0028-646X
DOI: 10.1111/nph.14102
Popis: Predictable seedling establishment is essential for resource-efficient and cost-effective crop production; it is widely accepted as a critically important trait determining yield and profitability. Seed vigour is essential to this, but its genetic basis is not understood.\ud We used natural variation and fine mapping in the crop Brassica oleracea to show that allelic variation at three loci influence the key vigour trait of rapid germination. Functional analysis in both B. oleracea and the model Arabidopsis identified and demonstrated activity of genes at these loci.\ud Two candidate genes were identified at the principal Speed of Germination QTL (SOG1) in B. oleracea. One gene BoLCVIG2 is a homologue of the alternative-splicing regulator (AtPTB1). The other gene BoLCVIG1 was unknown, but different alleles had different splice forms that were coincident with altered abscisic acid (ABA) sensitivity. We identified a further QTL, Reduced ABscisic Acid 1 (RABA1) that influenced ABA content and provide evidence that this results from the activity of a homologue of the ABA catabolic gene AtCYP707A2 at this locus.\ud Lines containing beneficial alleles of these three genes had greater seed vigour. We propose a mechanism in which both seed ABA content and sensitivity to it determines speed of germination.
Databáze: OpenAIRE
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