Insect immunity: developmental and inducible activity of the Drosophila diptericin promoter
| Autor: | D. Hoffmann, Jean-Luc Dimarcq, Daniel Zachary, Marie Meister, C Ruiz, Jean-Marc Reichhart, Jules A. Hoffmann, G Richards |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Embryo
Nonmammalian animal structures Recombinant Fusion Proteins Molecular Sequence Data Restriction Mapping Biology General Biochemistry Genetics and Molecular Biology Fusion gene Transcription (biology) Complementary DNA Drosophilidae Animals Drosophila Proteins Amino Acid Sequence Promoter Regions Genetic Molecular Biology Gene Antibacterial agent Mammals Regulation of gene expression Genetics Base Sequence General Immunology and Microbiology General Neuroscience fungi beta-Galactosidase biology.organism_classification Adipose Tissue Gene Expression Regulation Oligodeoxyribonucleotides Insect Hormones Insect Proteins Drosophila Drosophila Protein Acute-Phase Proteins Research Article |
| Zdroj: | The EMBO Journal. 11:1469-1477 |
| ISSN: | 0261-4189 |
| DOI: | 10.1002/j.1460-2075.1992.tb05191.x |
| Popis: | Diptericins are 9 kDa inducible antibacterial peptides initially isolated from immune haemolymph of Phormia (Diptera). Following the isolation of a Drosophila cDNA encoding a diptericin homologue, we have now cloned a genomic fragment containing the Drosophila diptericin gene. To dissect the regulation of this gene, we have transformed flies with a fusion gene in which the reporter beta-galactosidase gene is under the control of 2.2 kb upstream sequences of the diptericin gene. We show that such a fusion gene is inducible by injection of live bacteria or complete Freund's adjuvant and respects the tissue specific expression pattern of the resident diptericin gene. Our analysis reveals at least four distinct phases in the regulation of this gene: young larvae, late third instar larvae, pupae and adults. This complexity may be related to the presence in the upstream sequences of multiple copies of response elements previously characterized in genes encoding acute phase response proteins in mammals (e.g. NK-kappa B, NF-kappa B related, NF-IL6 response elements). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|