STRA6 Expression Serves as a Prognostic Biomarker of Gastric Cancer
Autor: | Masahiko Koike, Kouichi Sawaki, Kenji Omae, Masamichi Hayashi, Dai Shimizu, Shunsuke Nakamura, Suguru Yamada, Goro Nakayama, Norifumi Hattori, Mitsuro Kanda, Chie Tanaka, Yasuhiro Kodera |
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Rok vydání: | 2020 |
Předmět: |
Male
Cancer Research Microarray Retinoic acid Datasets as Topic Biochemistry Disease-Free Survival Cohort Studies Transcriptome 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Gastrectomy Risk Factors Stomach Neoplasms Cell Line Tumor Biomarkers Tumor Genetics Humans Medicine RNA-Seq Molecular Biology Aged Oligonucleotide Array Sequence Analysis Messenger RNA business.industry Stomach Retinol Membrane Proteins Cancer Middle Aged Prognosis medicine.disease Biomarker (cell) Survival Rate chemistry 030220 oncology & carcinogenesis Cancer research Female business RBP1 Research Article |
Zdroj: | Cancer Genomics Proteomics |
ISSN: | 1790-6245 1109-6535 |
Popis: | Background Despite advances in our understanding on the pathogenesis of gastric cancer (GC), patients face a poor prognosis. To improve clinical outcomes, effective approaches to diagnosis and treatment employing new diagnostic biomarkers are required to achieve early detection and predict recurrence and prognosis. Materials and methods Transcriptome analysis was conducted using surgically resected gastric tissues from four patients with metastatic GC. A total of 228 pairs of primary GC tissues and corresponding normal adjacent tissues were subjected to mRNA expression analysis. To validate our findings, we accessed an integrated microarray dataset and RNA sequencing data of GC cell lines. Results We identified stimulated by retinoic acid 6 (STRA6) as a differentially overexpressed gene, which encodes a transmembrane protein that mediates the cellular uptake of retinol. To investigate how STRA6 contributes to the malignant phenotype of GC cells, we mined public datasets and found the mRNA encoding retinol binding protein 1 (RBP1), which is associated with retinoid metabolism, was co-expressed with STRA6. Furthermore, STRA6 mRNA levels were significantly higher in GC tissues compared to the corresponding noncancerous adjacent tissues of 228 surgically resected gastric tissue samples. Moreover, patients with high levels of STRA6 mRNA experienced significantly shorter disease-free survival and overall survival. Multivariate analysis revealed that high levels of STRA6 served as a significant risk factor. Conclusion Patients with high levels of STRA6 mRNA experienced significantly worse clinical outcomes, indicating that STRA6 may serve as a diagnostic and prognostic biomarker of GC. |
Databáze: | OpenAIRE |
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