A specific inhibitor of cysteine proteases impairs a Vif-dependent modification of human immunodeficiency virus type 1 Env protein
| Autor: | Bruno Guy, J P Lecocq, K Dott, Michel Geist, Danièle Spehner, Marie-Paule Kieny |
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| Rok vydání: | 1991 |
| Předmět: |
Proteases
Gene Products vif Genes Viral viruses Blotting Western Molecular Sequence Data Immunology Cysteine Proteinase Inhibitors Biology Transfection Gp41 Antiviral Agents Microbiology Cell Line Viral Envelope Proteins Viral envelope Leucine Sequence Homology Nucleic Acid Virology Escherichia coli vif Gene Products Human Immunodeficiency Virus Animals Amino Acid Sequence Cloning Molecular Peptide sequence Genes vif Infectivity Base Sequence virus diseases biochemical phenomena metabolism and nutrition Molecular biology Cysteine Endopeptidases Insect Science HIV-1 Mutagenesis Site-Directed Oligonucleotide Probes Research Article Cysteine |
| Zdroj: | Journal of Virology. 65:1325-1331 |
| ISSN: | 1098-5514 0022-538X |
| Popis: | The Vif protein of human immunodeficiency virus type 1 (HIV-1) regulates viral infectivity. Virions produced in cell culture after transfection by a Vif-negative molecular clone show a dramatic decrease in infectivity for susceptible CD4+ cell lines, although the Vif protein does not appear to be a constituent of the viral particle. The exact mechanism by which Vif affects HIV-1 infectivity is so far unknown. We report the existence of structural homologies between Vif and a family of cysteine proteases and present evidence which suggests that one of the targets of Vif is the Env protein and more precisely the cytoplasmic domain of gp41. Vif was found to modify both the processing and conformation of the Env protein. Ethyl(25, 35)- 3[(5)-3-methyl-1-(3-methylbutylcarbamoyl)]oxirane-2-carboxylate, a specific inhibitor of cysteine proteases, inhibits the effect of Vif, as does the mutation of Cys-114 to Leu in Vif. Furthermore, Cys-114 of Vif produced in Escherichia coli, interacts directly with trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane. These observations suggest that a cysteine protease activity is associated with Vif and that this activity plays a role in Env maturation. |
| Databáze: | OpenAIRE |
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