Nitric oxide-mediated activation of extracellular signal-regulated kinase (erk) and c-jun n-terminal kinase (jnk) during hypoxia in cerebral cortical nuclei of newborn piglets
| Autor: | Alan B. Zubrow, Om P. Mishra, Qazi M. Ashraf |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Cerebral Cortex
MAPK/ERK pathway Swine Kinase General Neuroscience c-jun JNK Mitogen-Activated Protein Kinases Biology Nitric Oxide Molecular biology Nitric oxide Phosphocreatine Enzyme Activation chemistry.chemical_compound Animals Newborn chemistry Biochemistry Cell surface receptor Mitogen-activated protein kinase biology.protein Animals Phosphorylation Enzyme Inhibitors Mitogen-Activated Protein Kinases Hypoxia Brain |
| Zdroj: | Neuroscience. 123:179-186 |
| ISSN: | 0306-4522 |
| DOI: | 10.1016/j.neuroscience.2003.08.008 |
| Popis: | Previous studies have shown that mitogen-activated protein kinases, such as extracellular signal-related kinase (ERK) and c-Jun N-terminal kinase (JNK), mediate signal transduction from cell surface receptors to the nucleus and phosphorylate anti-apoptotic proteins thereby regulating programmed cell death. The present study tests the hypotheses that hypoxia activates ERK and JNK in neuronal nuclei of newborn piglets and the hypoxia-induced activation of ERK and JNK is mediated by nitric oxide (NO). Activated ERK and JNK were assessed by determining phosphorylated ERK and JNK using immunoblotting in six normoxic (Nx) and 10 hypoxic (Hx) and five N-nitro-L-arginine (a NOS inhibitor, 40 mg/kg,) -pretreated hypoxic (N-nitro-L-arginine [NNLA]-Hx) 3-5 day old piglets. Hypoxia was induced by decreasing inspired oxygen from 21% to 7% for 60 min. Cerebral tissue hypoxia was documented biochemically by determining the tissue levels of ATP and phosphocreatine (PCr). Cortical neuronal nuclei were isolated and the nuclear protein was analyzed for activated ERK and JNK using anti-phosphorylated ERK and JNK antibodies. Protein bands were detected using enhanced chemiluminescence method and analyzed by imaging densitometry. Protein density was expressed as absorbance ODxmm(2). ATP levels were 4.57+/-0.45 micromoles/g brain in the Nx group, 1.29+/-0.23 micromoles/g brain in the Hx group (P |
| Databáze: | OpenAIRE |
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