Significance of dynamic changes in gastric smooth muscle cell apoptosis, PI3K-AKT-mTOR and AMPK-mTOR signaling in a rat model of diabetic gastroparesis

Autor: Zheng Jin, Mo‑Han Zhang, Ying‑Lan Cai, Li‑Hua Piao, Jing‑Zhi Jiang
Rok vydání: 2017
Předmět:
Male
0301 basic medicine
Cancer Research
medicine.medical_specialty
Gastroparesis
Myocytes
Smooth Muscle
Apoptosis
P70-S6 Kinase 1
AMP-Activated Protein Kinases
Biochemistry
Diabetes Mellitus
Experimental
Rats
Sprague-Dawley
Ribosomal s6 kinase
Phosphatidylinositol 3-Kinases
03 medical and health sciences
0302 clinical medicine
Internal medicine
Tuberous Sclerosis Complex 2 Protein
Genetics
medicine
Animals
Myocyte
Phosphorylation
Molecular Biology
Protein kinase B
PI3K/AKT/mTOR pathway
biology
TOR Serine-Threonine Kinases
Tumor Suppressor Proteins
Stomach
Intracellular Signaling Peptides and Proteins
Ribosomal Protein S6 Kinases
70-kDa
AMPK
Phosphoproteins
medicine.disease
Disease Models
Animal
030104 developmental biology
Endocrinology
Oncology
030220 oncology & carcinogenesis
biology.protein
Molecular Medicine
Signal transduction
Carrier Proteins
Proto-Oncogene Proteins c-akt
Signal Transduction
Zdroj: Molecular Medicine Reports. 16:1530-1536
ISSN: 1791-3004
1791-2997
DOI: 10.3892/mmr.2017.6764
Popis: The aim of the present study was to investigate the significance of cell apoptosis, the phosphoinositide-3-kinase (PI3K)-protein kinase B (AKT)-mammalian target of rapamycin (mTOR) pathway, and the 5' adenosine monophosphate-activated protein kinase (AMPK)‑mTOR pathways in the process of diabetic gastroparesis. Changes in gastric smooth muscle cells of diabetic rats with induced gastroparesis were examined. The diabetic rat model was established by dividing animals into a normal control group and diabetic model groups examined at 2, 4 and 6 weeks. Diabetic gastroparesis was evaluated by examining the rates of gastric residual pigment, whereas flow cytometry was used to detect the apoptosis of gastric smooth muscle cells. The expression levels of PI3K and phosphorylated (p‑) AKT, AMPK, mTOR, tuberous sclerosis complex 2, p70 ribosomal S6 kinase, and eukaryotic translation initiation factor 4‑binding protein 1 were determined in gastric muscles using western blot analysis. Diabetic gastroparesis was confirmed in models at 6 weeks. The apoptosis of gastric smooth muscle cells gradually increased in all diabetic groups, and significant changes were observed in key proteins involved in PI3K‑AKT‑mTOR and AMPK‑mTOR signaling. The results indicated that apoptosis was important in the occurrence of diabetic gastroparesis, and the PI3K‑AKT‑mTOR and AMPK‑mTOR pathways were activated during the apoptotic processes, but were incapable of regulating apoptosis.
Databáze: OpenAIRE
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