Expression of pituitary tumor-transforming 2 in human glioblastoma cell lines and its role in glioblastoma tumorigenesis
| Autor: | Song-Bai Xu, Yi-Meng Shao, Yunbao Guo, Jing Chen, Xing-Dong Zhang, Hai-Yan Liu |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cancer Research Matrigel medicine.diagnostic_test Cell growth Cell Articles General Medicine Cell cycle Biology Molecular biology Flow cytometry 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Immunology and Microbiology (miscellaneous) chemistry Lipofectamine Apoptosis 030220 oncology & carcinogenesis medicine Propidium iodide |
| Zdroj: | Experimental and Therapeutic Medicine. 11:1847-1852 |
| ISSN: | 1792-1015 1792-0981 |
| DOI: | 10.3892/etm.2016.3159 |
| Popis: | The present study aimed to investigate the association between the expression of pituitary tumor-transforming 2 (PTTG2), and cell proliferation, invasion and apoptosis in glioblastoma. The U251 human glioblastoma cell line was transfected with the pcDNA-PTTG2 and small interfering (si)RNA-PTTG2 plasmids using Lipofectamine 2000. The expression of PTTG2 in U251 glioblastoma cells was determined using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot analysis. The association between PTTG2 expression, and cell proliferation, invasion and apoptosis in vitro were investigated using an MTT assay, Matrigel Transwell assay and flow cytometry combined with Annexin V/propidium iodide staining, respectively. RT-qPCR and western blot analysis demonstrated that PTTG2 mRNA and protein expression were significantly overexpressed and significantly suppressed following transfection with pcDNA-PTTG2 and short interfering RNA (siRNA)-PTTG2 plasmids, respectively (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|