A novel automated image analysis method for accurate adipocyte quantification
| Autor: | Michael A. Cawthorne, Osman S. Osman, Jacqueline F. O’Dowd, Joanne L. Selway, Kenneth Langlands, Jonathan R.S. Arch, Sabah Jassim, Claire J. Stocker, Malgorzata A. Kępczyńska |
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| Rok vydání: | 2013 |
| Předmět: |
high fat
Histology business.industry Adipose tissue Image processing Cell Biology adipocyte histomorphometry Cell size chemistry.chemical_compound chemistry paraffin embedding image analysis Murine model Adipocyte Paraffin section Medicine Metabolic disease diet business Analysis method Research Paper automation Biomedical engineering |
| Zdroj: | Adipocyte |
| ISSN: | 2162-397X 2162-3945 |
| DOI: | 10.4161/adip.24652 |
| Popis: | Increased adipocyte size and number are associated with many of the adverse effects observed in metabolic disease states. While methods to quantify such changes in the adipocyte are of scientific and clinical interest, manual methods to determine adipocyte size are both laborious and intractable to large scale investigations. Moreover, existing computational methods are not fully automated. We, therefore, developed a novel automatic method to provide accurate measurements of the cross-sectional area of adipocytes in histological sections, allowing rapid high-throughput quantification of fat cell size and number. Photomicrographs of H&E-stained paraffin sections of murine gonadal adipose were transformed using standard image processing/analysis algorithms to reduce background and enhance edge-detection. This allowed the isolation of individual adipocytes from which their area could be calculated. Performance was compared with manual measurements made from the same images, in which adipocyte area was calculated from estimates of the major and minor axes of individual adipocytes. Both methods identified an increase in mean adipocyte size in a murine model of obesity, with good concordance, although the calculation used to identify cell area from manual measurements was found to consistently over-estimate cell size. Here we report an accurate method to determine adipocyte area in histological sections that provides a considerable time saving over manual methods. |
| Databáze: | OpenAIRE |
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