Assessing the Use of p16INK4a Promoter Gene Methylation in Serum for Detection of Bladder Cancer
| Autor: | Francisco Javier Oliver, A Zuluaga, M. T. Valenzuela, M. Villalobos, J M Ruiz de Almodóvar, María Isabel Núñez, R Galisteo |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Oncology Pathology medicine.medical_specialty Urology Sensitivity and Specificity Internal medicine Biomarkers Tumor medicine Humans Promoter Regions Genetic Cyclin-Dependent Kinase Inhibitor p16 Aged Bladder cancer Urinary bladder business.industry Carcinoma in situ Case-control study Cancer Methylation DNA Methylation medicine.disease medicine.anatomical_structure Urinary Bladder Neoplasms DNA methylation Biomarker (medicine) Female business |
| Zdroj: | European Urology. 42:622-630 |
| ISSN: | 0302-2838 |
| DOI: | 10.1016/s0302-2838(02)00468-2 |
| Popis: | Objective: This study was undertaken to investigate whether hypermethylation in p16 INK4a gene promoter could serve as plasma biomarker of bladder cancer. Methods and Patients: We examined the p16 INK4a status using methylation-specific PCR in 86 cancer patients and 49 controls (31 healthy people and 18 patients with benign urological diseases). Results: The p16 INK4a methylation was found in 22% of the serum samples and in 26% of the bladder cancer biopsies; one of them with carcinoma in situ. The presence of hypermethylated p16 INK4a in serum seems to be a product from tumour cells because a strong statistical association was found between both matched DNA signals (p < 0:0001). Using the control group, the presence of methylated p16 INK4a in the serum of individuals with suspicion of bladder cancer was found to be associated with the tumour presence (p ¼ 0:0009). Aberrant p16 INK4a methylation was also observed in one non-cancer patient, which is undergoing further assessment. Conclusions: According with our results, methylation of p16 INK4a promoter may be involved in the bladder cancer genesis and the presence of p16 INK4a methylated in serum of these patients could be useful in the cancer diagnosis with values of sensitivity, specificity and positive predictive value of 0.226, 0.950 and 0.98, respectively. These figures support the use of methylated p16 INK4a as a new class of tumour marker in bladder cancer. |
| Databáze: | OpenAIRE |
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