Molecular characterization of double-stranded RNA virus in Trichomonas vaginalis Egyptian isolates and its association with pathogenicity
| Autor: | Amira B Mokhtar, Eman K El-Gayar, Wael Abdo Hassan |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Virulence medicine.disease_cause Asymptomatic Virus 03 medical and health sciences Mice Medical microbiology medicine Trichomonas vaginalis Parasite hosting Animals Humans RNA Viruses RNA Double-Stranded Mice Inbred BALB C Trichomoniasis General Veterinary biology General Medicine biology.organism_classification medicine.disease Virology Disease Models Animal 030104 developmental biology Infectious Diseases Insect Science Double-stranded RNA viruses Parasitology Egypt Female medicine.symptom Trichomonas Vaginitis |
| Zdroj: | Parasitology research. 115(10) |
| ISSN: | 1432-1955 |
| Popis: | Trichomoniasis is a common human sexually transmitted infection caused by Trichomonas vaginalis. The parasite can be infected with double-stranded RNA viruses (TVV). This viral infection may have important implications on trichomonal virulence and disease pathogenesis. This study aimed to determine the prevalence of T. vaginalis virus among isolates obtained from infected (symptomatic and asymptomatic) women in Ismailia City, Egypt, and to correlate the virus-infected isolates with the clinical manifestations of patients. In addition, the pathogenicity of TVV infected isolates on mice was also evaluated. T. vaginalis isolates were obtained from symptomatic and asymptomatic female patients followed by axenic cultivation in Diamond's TYM medium. The presence of T. vaginalis virus was determined from total extraction of nucleic acids (DNA-RNA) followed by reverse transcriptase-PCR. Representative samples were inoculated intraperitoneally in female albino/BALB mice to assess the pathogenicity of different isolates. A total of 110 women were examined; 40 (36.3 %) samples were positive for T. vaginalis infection. Of these 40 isolates, 8 (20 %) were infected by TVV. Five isolates contained TVV-2 virus species, and the remaining three isolates were infected withTVV-4 variant. A significant association was found between the presence of TVV and particular clinical manifestations of trichomoniasis. Experimental mice infection showed varying degrees of pathogenicity. This is the first report on T. vaginalis infection by TVV in Egypt. The strong association detected between TVV and particular clinical features of trichomoniasis and also the degree of pathogenicity in experimentally infected mice may indicate a possible clinical significance of TVV infection of T. vaginalis isolates. |
| Databáze: | OpenAIRE |
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