Induction of Metastasis by S100P in a Rat Mammary Model and Its Association with Poor Survival of Breast Cancer Patients
| Autor: | Philip S. Rudland, Joe Carroll, Roger Barraclough, John H. R. Winstanley, Angela Platt-Higgins, Suzete de Silva Rudland, Guozheng Wang |
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| Rok vydání: | 2006 |
| Předmět: |
Adult
Cancer Research Pathology medicine.medical_specialty Sialoglycoproteins Mammary gland Breast Neoplasms Mammary Neoplasms Animal Transfection Risk Assessment S100 protein Metastasis Breast cancer Tumor Cells Cultured Carcinoma medicine Animals Humans Neoplasm Invasiveness S100 Calcium-Binding Protein A4 Osteopontin Neoplasm Metastasis Rats Wistar Aged Aged 80 and over biology Calcium-Binding Proteins S100 Proteins Cancer Middle Aged Prognosis medicine.disease Immunohistochemistry Survival Analysis Neoplasm Proteins Rats medicine.anatomical_structure Oncology Antibody Formation Multivariate Analysis biology.protein Cancer research Female Antibody Follow-Up Studies |
| Zdroj: | Cancer Research. 66:1199-1207 |
| ISSN: | 1538-7445 0008-5472 |
| DOI: | 10.1158/0008-5472.can-05-2605 |
| Popis: | S100P, an EF-hand calcium-binding protein, has been reported to be associated with the progression of many types of cancers. Transfection of an expression vector for S100P into a benign, nonmetastatic rat mammary cell line causes a 4- to 6-fold increase in its level in all four transformant cell clones. When the resultant transformant cell lines are introduced in turn into the mammary fat pads of syngeneic Furth-Wistar rats, there is a significant 3-fold increase in local muscle invasion and a significant induction of metastasis in 64% to 75% of tumor-bearing animals. In a group of 303 breast cancer patients followed for up to 20 years, antibodies to S100P immunocytochemically stain 161 primary tumors. Survival of patients with S100P-positive carcinomas is significantly worse by about 7-fold than for those with negatively stained carcinomas. There is also a significant association between the class level of immunocytochemical staining of the carcinoma cells and decreased patient survival. Positive staining for S100P is significantly associated with that for two other metastasis-inducing proteins, S100A4 and osteopontin. Patients with tumors that stained positively for both S100P and S100A4 have a significantly reduced survival of 1.1% over patients with either S100 protein alone. Multivariate regression analysis identifies S100P, S100A4, and osteopontin as the most significant independent indicators of death in this group of patients. These results suggest that stratification of patients into groups according to expression of multiple metastasis-inducing proteins may lead to a more accurate prediction of patient survival. (Cancer Res 2006; 66(2): 1199-207) |
| Databáze: | OpenAIRE |
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