Improvement of androgenetic alopecia with topicalSophora flavescensAiton extract, and identification of the two active compounds in the extract that stimulate proliferation of human hair keratinocytes
| Autor: | T. Iwabuchi, M. Tajima, Y. Nakazawa, A. Ishino, T. Takahashi, C. Hamada, T. Arai |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Keratinocytes Male 0301 basic medicine medicine.medical_specialty Pterocarpans Dermatology Biology Pharmacology Organ culture Plant Roots 030207 dermatology & venereal diseases 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Humans Medicarpin Cell Proliferation Sophora flavescens integumentary system Plant Extracts Cell growth Pterocarpan Alopecia Fibroblasts Middle Aged Hair follicle biology.organism_classification 030104 developmental biology Endocrinology medicine.anatomical_structure chemistry Keratinocyte Hair Follicle Sophora Ex vivo Hair |
| Zdroj: | Clinical and Experimental Dermatology. 41:302-307 |
| ISSN: | 0307-6938 |
| DOI: | 10.1111/ced.12753 |
| Popis: | SummaryBackground Androgenetic alopecia (AGA) is a hair loss disorder that commonly affects middle-aged men. To date, the properties of a number of natural or synthetic substances have been investigated for their ability to improve the condition. Aim To evaluate the hair growth-promoting activities of an extract from the root of Sophora flavescens Aiton. Methods We used a human hair keratinocyte proliferation assay and ex vivo organ cultures of human hair follicle to examine the potential of the extract to stimulate hair growth via anagen elongation. We isolated the compounds promoting the growth of epithelial cells, and determined their chemical structures. A randomized, double-blinded, placebo-controlled clinical study for S. flavescens extract was carried out for 6 months with patients with AGA. Results The extract stimulated the proliferation of hair keratinocytes at a concentration of 0.1 ng/mL, while 100 ng/mL of the extract had a marked effect on hair shaft elongation in an organ culture of human hair follicle. Cell proliferation assay-directed fractionation led to the identification of two pterocarpan derivatives, L-maackiain and medicarpin, as active compounds that promote the proliferation of human hair keratinocytes. Studies in human subjects showed that improvement in the inspected alopecia scores in the lotion plus extract group were significant over a period of 6 months (P < 0.01). Conclusions S. flavescens root extract is effective for the treatment of AGA. The isolated two pterocarpans might have important role in this effect. |
| Databáze: | OpenAIRE |
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