Studies on platelets contained in eluates following filtration leukapheresis

Autor:	D. Dubovsky, D Shackleton, L. M. Kernoff, K Blake, Peter Jacobs
Rok vydání:	1979
Předmět:	Blood Platelets Chromatography Chemistry Nylon fiber Immunology Hematology Leukapheresis Granulocyte law.invention medicine.anatomical_structure In vivo law medicine Humans Immunology and Allergy Platelet Normal control Filtration
Zdroj:	Transfusion. 19:114-119
ISSN:	1537-2995 0041-1132
DOI:	10.1046/j.1537-2995.1979.19279160279.x
Popis:	Platelets eluted from nylon fiber filters after filtration leukapheresis have been studied. The platelet yield from 61 routine donations was 1.25 +/- 0.18 x 10(11), (mean +/- SEM) corresponding to 1.78 x 10(10) per 500 ml blood processed. Filtered platelets labeled with radiochromate demonstrated reduced recovery in vivo 15 minutes after infusion (38.5 +/- 1.7%) when compared to the control value (68.5 +/- 6.8, p less than 0.001). The survival of those platelets remaining in the circulation after 15 minutes did not however differ from the control value. ADP (10 micrometer, 1 mM), adrenaline (100 micrometer) and collagen (7.25 mg/ml) added in vitro induced less aggregation of filtered platelets than normal control platelets and electron microscopy revealed structural abnormalities. It is concluded that recipients of granulocyte transfusions obtained by filtration leukapheresis are unlikely to be benefited by the platelets contained in these transfusions.
Databáze:	OpenAIRE
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