Generation and characterization of a human induced pluripotent stem (iPS) cell line derived from an acute myeloid leukemia patient evolving from primary myelofibrosis carrying the CALR 52 bp deletion and the ASXL1 p.R693X mutation
| Autor: | Jaroslaw Sochacki, Rodrigo Madeiro da Costa, Stevens K. Rehen, Mayra Carneiro, Telma Padilha, Marcelo Reis, Bárbara C.R. Monte-Mór, Mariana D'Andrea, Adelmo Daumas, Martín Hernán Bonamino, Cintia Elisabeth Gomez Limia, Sylvie Devalle, Ilana Zalcberg, Cristiana Solza |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Myeloid Cellular differentiation Induced Pluripotent Stem Cells Biology medicine.disease_cause Cell Line Kruppel-Like Factor 4 03 medical and health sciences medicine Animals Humans Myelofibrosis Induced pluripotent stem cell lcsh:QH301-705.5 Mutation Myeloid leukemia Cell Differentiation Cell Biology General Medicine Middle Aged medicine.disease Leukemia Myeloid Acute Leukemia 030104 developmental biology medicine.anatomical_structure lcsh:Biology (General) Primary Myelofibrosis Cancer research Female Reprogramming Developmental Biology |
| Zdroj: | Stem Cell Research, Vol 24, Iss C, Pp 16-20 (2017) |
| ISSN: | 1876-7753 1873-5061 |
| Popis: | Peripheral blood sample was donated by a 61 years old female patient diagnosed with acute myeloid leukemia secondary to a primary myelofibrosis harboring the 52-bp deletion in the CALR gene (c.1092_1143del, p.L367fs*46) and the R693X mutation in the ASXL1 gene (c.2077C>T, p.R693X). CD34+ cells were isolated from the sample and subjected to the reprogramming procedure by using the Sendai virus carrying the reprogramming factors Oct3/4, Sox2, Klf4 and c-Myc. iPS colonies generated retained the original mutations and displayed all the features of bona fide iPS cells. |
| Databáze: | OpenAIRE |
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