Silencing and Heterologous Expression of ppo-2 Indicate a Specific Function of a Single Polyphenol Oxidase Isoform in Resistance of Dandelion (Taraxacum officinale) Against Pseudomonas syringae pv. tomato
| Autor: | Christian Schulze Gronover, Mareike E. Dirks, Dirk Prüfer, Bruno M. Moerschbacher, Carolin G. Richter |
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| Rok vydání: | 2012 |
| Předmět: |
DNA
Complementary Time Factors Taraxacum Physiology Arabidopsis Pseudomonas syringae Dandelion Genetically modified crops Plant disease resistance Microbiology Anti-Infective Agents Taraxacum officinale Gene Expression Regulation Plant Botany Arabidopsis thaliana Gene Silencing Transgenes Promoter Regions Genetic Disease Resistance Plant Diseases Plant Proteins Botrytis cinerea Molecular Structure biology Plant Extracts fungi food and beverages General Medicine Plants Genetically Modified biology.organism_classification Isoenzymes Plant Leaves RNA Plant RNA Interference Botrytis Heterologous expression Agronomy and Crop Science Catechol Oxidase |
| Zdroj: | Molecular Plant-Microbe Interactions®. 25:200-210 |
| ISSN: | 1943-7706 0894-0282 |
| DOI: | 10.1094/mpmi-04-11-0082 |
| Popis: | Dandelion (Taraxacum officinale) possesses an unusually high degree of disease resistance. As this plant exhibits high polyphenol oxidase (PPO) activity and PPO have been implicated in resistance against pests and pathogens, we analyzed the potential involvement of five PPO isoenzymes in the resistance of dandelion against Botrytis cinerea and Pseudomonas syringae pv. tomato. Only one PPO (ppo-2) was induced during infection, and ppo-2 promoter and β-glucuronidase marker gene fusions revealed strong induction of the gene surrounding lesions induced by B. cinerea. Specific RNAi silencing reduced ppo-2 expression only, and concomitantly increased plant susceptibility to P. syringae pv. tomato. At 4 days postinoculation, P. syringae pv. tomato populations were strongly increased in the ppo-2 RNAi lines compared with wild-type plants. When the dandelion ppo-2 gene was expressed in Arabidopsis thaliana, a plant having no PPO gene, active protein was formed and protein extracts of the transgenic plants exhibited substrate-dependent antimicrobial activity against P. syringae pv. tomato. These results clearly indicate a strong contribution of a specific, single PPO isoform to disease resistance. Therefore, we propose that specific PPO isoenzymes be included in a new family of pathogenesis-related (PR) proteins. |
| Databáze: | OpenAIRE |
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