Kinetics of superoxide production by stimulated neutrophils
Autor: | C. Murali Krishna, Amram Samuni, Harry L. Malech, Christopher D.V. Black, Angelo Russo, Dwight Kaufman, John A. Cook |
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Rok vydání: | 1991 |
Předmět: |
Granulocyte activation
Neutrophils Kinetics Biophysics Stimulation Ferricytochrome c In Vitro Techniques Biochemistry law.invention chemistry.chemical_compound Biosynthesis law Superoxides Humans Molecular Biology Chemiluminescence Peroxidase Chemistry Superoxide Electron Spin Resonance Spectroscopy Superoxide radical Luminescent Measurements Tetradecanoylphorbol Acetate Granulocytes |
Zdroj: | Archives of biochemistry and biophysics. 286(1) |
ISSN: | 0003-9861 |
Popis: | Oxygen-derived active species and superoxide radical in particular are generated and excreted upon granulocyte activation and are instrumental in host defense against bacterial and fungal infections. Associated with the activation of neutrophils is an apparent transitory oxy-radical production. Evidence from independent methods has previously suggested that radical production peaks shortly following neutrophil stimulation and decays within minutes. However, since neutrophil function in the body might reasonably be expected to last beyond the few minutes following stimulation, cessation of the production of oxy-radicals is unexpected. In an attempt to reconcile this discrepancy, the formation kinetics of superoxide by stimulated human neutrophils was reinvestigated by three independent methods: electron spin resonance, chemiluminescence, and ferricytochrome c reduction. The present results demonstrate that under appropriate experimental conditions stimulated neutrophils have the capacity to produce superoxide for several hours. The reasons for the previously reported “apparent” ephemeral nature of oxy-radical formation are discussed. |
Databáze: | OpenAIRE |
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