Quantifying intracellularMycobacterium tuberculosis: An essential issue for in vitro assays
| Autor: | Luz Mary Salazar, Marisol Ocampo, Manuel A. Patarroyo, Deisy Carolina Rodriguez |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Tuberculosis bacterial quantification Fluorescent Antibody Technique Review Computational biology infection assay Biology Protein degradation Polymerase Chain Reaction Microbiology Mycobacterium tuberculosis 03 medical and health sciences Multiplicity of infection Immune system medicine Humans Tuberculosis Pulmonary Pathogen Staining and Labeling 030102 biochemistry & molecular biology Macrophages In vitro toxicology Flow Cytometry cytometry medicine.disease biology.organism_classification Bacterial Load 030104 developmental biology tuberculosis Host-Pathogen Interactions fluorescence Intracellular |
| Zdroj: | MicrobiologyOpen |
| ISSN: | 2045-8827 |
| DOI: | 10.1002/mbo3.588 |
| Popis: | Many studies about intracellular microorganisms which are important regarding diseases affecting public health have been focused on the recognition of host–pathogen interactions, thereby ascertaining the mechanisms by which the pathogen invades a cell and makes it become its host. Such knowledge enables understanding the immunological response triggered by these interactions for obtaining useful information for developing vaccines and drugs. Quantitative cell infection assay protocols are indispensable regarding studies involving Mycobacterium tuberculosis, which takes the lives of more than 2 million people worldwide every year; however, sometimes these are limited by the pathogen's slow growth. Concerning such limitation, a detailed review is presented here regarding the different methods for quantifying and differentiating an intracellular pathogen, the importance of mycobacteria aggregate dissociation and multiplicity of infection (MOI) in infection assays. The methods’ differences, advantages, and disadvantages are discussed regarding intra and extracellular bacteria (on cell surface) differentiation, current problems are outlined, as are the solutions provided using fluorophores and projections made concerning quantitative infection assays. |
| Databáze: | OpenAIRE |
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